Protocol for Creating Microfluidic Bioreactors

1. Clean silicon wafer with nitrogen gun

2. Make PDMS

a. Weigh out silicone elastomer base in a 10:1 ratio by weight against silicone elastomer curing agent in polystyrene cup.

b. Mix thoroughly.

c. Degas mixture using vacuum.

3. Pour roughly 40g of PDMS into each dish containing a wafer.

4. Remove all bubble formations using sharp tip.

5. Place in 70°C oven for at least two hours.

6. Cut out PDMS impression avoiding protrusions.

7. Using sharp-tip needle, puncture holes at each inlet and outlet.

8. Plasma clean PDMS and glass slides to bind them irreversibly, and attach as follows:

9. Insert blunt-end needles into holes created at inlets and outlets.

10. Place cylindrical support around needle and fill with epoxy, being careful not to get epoxy on needle tip.