1. Clean silicon wafer with nitrogen gun
2. Make PDMS
a. Weigh out silicone elastomer base in a 10:1 ratio by weight against silicone elastomer curing agent in polystyrene cup.
b. Mix thoroughly.
c. Degas mixture using vacuum.
3. Pour roughly 40g of PDMS into each dish containing a wafer.
4. Remove all bubble formations using sharp tip.
5. Place in 70°C oven for at least two hours.
6. Cut out PDMS impression avoiding protrusions.
7. Using sharp-tip needle, puncture holes at each inlet and outlet.
8. Plasma clean PDMS and glass slides to bind them irreversibly, and attach as follows:
9. Insert blunt-end needles into holes created at inlets and outlets.
10. Place cylindrical support around needle and fill with epoxy, being careful not to get epoxy on needle tip.