© Weigh out 0.1 g gelatin and place into a 500 ml glass bottle.
© Add distilled water to the 500 ml mark, and autoclave. This gelatin will go into solution while being autoclaved. The concentration of gelatin in 0.02%.
© Fibronectin is received in a tube as 5 ml liquid. Dilute 1 ml fibronectin in 199 ml of 0.02% gelatin. Mix gently, and immediately aliquot 6 ml per 15 ml centrifuge tube. Freeze aliquots at -20°C.
© Before culturing cells, coat tissue culture flasks with gelatin/fibronectin (2 ml/T25 or 6 ml/T75 flask). Cap the flasks, and incubate at 37°C overnight.
© Remove the gelatin/fibronectin by aspiration the next day just before adding cells to the flasks.