Synthesis of Acr-PEG-Peptide/Growth Factor

    1. Determine how much Acr-PEG-stuff you want/need and convert to moles

    2. Calculate needed amount of PEG(3400)-NHS (mol to g – MW ~3220)

    3. Determine how much of the pendant group to add – use 20% excess

    4. Prepare either a 50 mM Tris buffer, pH 8.5 or a 50 mM sodium bicarbonate buffer, pH 8.4

    5. React PEG-NHS and pendant group in ~100 ml of buffer in a round bottom flask for 2 hours

    6. Pour liquid into a freeze-drying vessel and freeze in -80 ºC

    7. Freeze-dry

    8. Resuspend in PBS and dialyze in appropriately pore sized dialyzer for 3 days with 2 exchanges of PBS

    9. Freeze the dialyzed product again at -80 ºC

    10. Freeze-dry