DATE CREATED: 1/7/09
Project Goals
Creating material gradients with variances in mechanical and chemical properties by using microfluidic devices
Investigating the role of chemical and mechanical factors in angiogenesis
Things to look at:
Tubulogenesis
Experimental Overview
Create chemical and mechanical gradients in two separate microfluidic devices (using passive pump)
Use HUVEC, and treat with VEGF
Examine the differences in angiogenesis
Possible Assays:
Seed cells on 2D glass substrate, form 3D gel over the cells, treat with VEGF, investigate the cell penetration into the matrix
Seed cells in 3D gel, treat with VEGF, investigate the effect on the individual cells forming multi-cellular constructs
Seed cells on 2D glass substrate, form a gradient of VEGF, have cells migrate into a 3D matrix adjacent to the cell monolayer
May require the use of the three-inlet channel
ACTION PLAN
PROBLEMS IDENTIFIED (PI) / OUTSIDE SKILL REQUIRED (OSR) / RESOLVED (R)
PAPER TITLE
A) Background
B) Hypothesis
B) Specific Aims
C) General Experimental Approach (Design etc)
D) Pitfalls and alternatives
E) Potential Figures
F) Future Directions
A) BACKGROUND:
B) HYPOTHESIS
C) SPECIFIC AIMS
AIM 1 -
AIM 2 -
D) GENERAL EXPERIMENTAL APPROACH
E) PITFALLS AND ALTERNATIVES
F) ANTICIPATED FIGURES FOR PAPER or when you have data, FIGURES FOR PAPER
G) FUTURE DIRECTIONS
H) MATERIALS AND METHODS
I) RESULTS
J) DISCUSSION
K) LITERATURE