Angiogenesis study using chemical and mechanical material gradients

DATE CREATED: 1/7/09

Project Goals

    • Creating material gradients with variances in mechanical and chemical properties by using microfluidic devices

    • Investigating the role of chemical and mechanical factors in angiogenesis

    • Things to look at:

      • Tubulogenesis

Experimental Overview

  • Create chemical and mechanical gradients in two separate microfluidic devices (using passive pump)

    • Use HUVEC, and treat with VEGF

  • Examine the differences in angiogenesis

Possible Assays:

  • Seed cells on 2D glass substrate, form 3D gel over the cells, treat with VEGF, investigate the cell penetration into the matrix

  • Seed cells in 3D gel, treat with VEGF, investigate the effect on the individual cells forming multi-cellular constructs

  • Seed cells on 2D glass substrate, form a gradient of VEGF, have cells migrate into a 3D matrix adjacent to the cell monolayer

    • May require the use of the three-inlet channel

ACTION PLAN

PROBLEMS IDENTIFIED (PI) / OUTSIDE SKILL REQUIRED (OSR) / RESOLVED (R)

PAPER TITLE

A) Background

B) Hypothesis

B) Specific Aims

C) General Experimental Approach (Design etc)

D) Pitfalls and alternatives

E) Potential Figures

F) Future Directions

A) BACKGROUND:

B) HYPOTHESIS

C) SPECIFIC AIMS

AIM 1 -

AIM 2 -

D) GENERAL EXPERIMENTAL APPROACH

E) PITFALLS AND ALTERNATIVES

F) ANTICIPATED FIGURES FOR PAPER or when you have data, FIGURES FOR PAPER

G) FUTURE DIRECTIONS

H) MATERIALS AND METHODS

I) RESULTS

J) DISCUSSION

K) LITERATURE