Experiment Protocols
General Lab Maintenance
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Lab Procedures
Fluorescent Microscope Operation
Fluorescent Microscope Image Acquisition
PDMS - mixing and casting
Plasma Cleaning
TMSPMA coating of glass slides
Standard operation protocol for UV curing system (Omnicure S2000)
Cell and Tissue Culture Protocols
Poly-L-lysine coating of slides
Use of Hemacytometer to count cells
Tethering cells to glass coverslip
Pre-coating flasks
Passaging: this is the procedure for a 1:2 split
BioMEMs Protocols
Preparing Chitosan Nano/Microparticles
Protein Patterning using Parylene
Protocol for Creating Microfluidic Bioreactors
Making gradient solution using microfluidic device
Source of Chemicals for Tube manufacture
Procedure for manufacturing two hydrogel tubes
Procedure for Preparing Devices for Implantation.
Chemistry/Synthesis Protocols
Synthesis of Acr-PEG-Peptide/Growth Factor
Synthesis of Diacrylated/Dimethacrylated PE
Acryloyl-PEG-peptide preparation
How to prepare an X% PEG-DA Solution
Methacrylated Sebacic Acid (MSA) Preparation1
1,3-Bis(p-carboxyphenoxy) propane/hexane (CPP or CPH) Preparation
(1,3-Bis(p-carboxyphenoxy))propyl/hexyl Dimethacrylate1
Methacrylation of Hyaluronic Acid (HA)
Modified Protocol for making Bovine Fibrin (Sigma)
Analysis Protocols
Making collagen gel for embedding EBs
Sample Preparation for Cryosectioning
Binding Strength of Serum Proteins to Hyaluronic Acid Gels
Micro BCA Protein Assay Protocol (Pierce)
Determining intranuclear Expression by Flow Cytometry
Haematoxylin and Eosin Staining
Immunohistochemistry / Hematoxylin staining
Alkaline Phosphatase and Von Kossa Staining
Animal work