RNA Quantification

    1. Pour off ethanol from RNA sample carefully – let air dry 5 min.

    2. Add 100 μl 0.1% DEPC water, put samples in oven (55-60 ºC) for 10 min.

    3. Blank UV-vis (260 nm) with 0.1% DEPC water

    4. Prepare samples

      1. 990 μl of 0.1% DEPC water

      2. add 10 μl sample

      3. mix thoroughly

    5. To find volume to add (Vadd) to get 10 ng/μl:

      1. (OD260nm*4000*90 (resuspended concentration minus sample - Vs))/10 (wanted concentration) = V

      2. V-Vs = Vadd