RNA Quantification
Pour off ethanol from RNA sample carefully – let air dry 5 min.
Add 100 μl 0.1% DEPC water, put samples in oven (55-60 ºC) for 10 min.
Blank UV-vis (260 nm) with 0.1% DEPC water
Prepare samples
990 μl of 0.1% DEPC water
add 10 μl sample
mix thoroughly
To find volume to add (Vadd) to get 10 ng/μl:
(OD260nm*4000*90 (resuspended concentration minus sample - Vs))/10 (wanted concentration) = V
V-Vs = Vadd