2014MHS

蕭名宏 (Ming-Hong Siao)

Development of monolayer-contacted lipid bilayer platform applied to atomic force microscopy measurement

可配合原子力顯微鏡量測之雙層脂質膜成膜平台之開發

In this research, two designs of lipid bilayer formation method have been demonstrated for the goal of application of dynamically scanning membrane protein by atomic force microscopy. First, the hydrogel-droplet vertical bilayer formation system is utilized to form lipid bilayer. Although a bilayer is successfully formed by this system, there is no reasonable electrophysiology signal being measured and the lipid bilayer formation success rate is only 20%. It is not good enough for the application of atomic force microscopy dynamically scanning process. Besides, the oblique droplet interface bilayer formation system is also provided to lipid bilayer formation and the atomic force microscopy dynamically scanning. For the two aqueous droplets system, this method demonstrates lipid bilayer formation, membrane protein insertion and oblique lipid bilayer formation. In this system, success rate of the lipid bilayer formation is about 80% and the longest bilayer life time is about three hours. There is a step-liked current signal caused by the membrane protein insertion which provides an evidence of lipid bilayer existence. An oblique lipid bilayer formation experiment indicates that a droplet interface bilayer can be formed at 0∘to 45∘related to horizontal. In addition, this system provides a new method of droplet transporting. An aqueous droplet is mixed with magnetic fluid in a particular ratio, 1:300, so that the droplet can be transported by magnet rapidly before lipid bilayer formation. In this bilayer formation system, a charge/discharge signal of lipid bilayer capacitance is also been measured by inserting two electrodes into the droplets.