Embedded Files
邵啟煥 (Chi-Ahuan Shao)
邵啟煥 (Chi-Ahuan Shao)
A New Capillary Electrophoresis Microchip by Liquid Dielectrophoresis for DNA Analysis
A New Capillary Electrophoresis Microchip by Liquid Dielectrophoresis for DNA Analysis
以液介電泳原理開發應用於DNA分析之新式毛細管電泳晶片
以液介電泳原理開發應用於DNA分析之新式毛細管電泳晶片
Capillary electrophoresis (CE) microchip has played an important role in DNA analysis in recent years. However, all CE microchips nowadays are builted by close channel. We have developed a novel capillary electrophoresis microchip by an open channel technique without channel walls. The open channel here is created by utilizing liquid dielectrophoresis (LDEP) principle to confine liquids above the LDEP electrodes and form a open channel around the vertical AC electric field at 100 kHz, 100 V. While we applied a parallel DC electric field, electroosmotic flow (EOF) was observed by FITC doping in buffer solution. The device has demonstrated injection, separation and detection of DNA between two parallel plates. The sample can be transported by electroosmotic flow at parallel DC electric field. DNA was preliminary analyzed at DC electric field with amplitude of 26.6 V/cm in an open channel micro CE chip by fluorescence microscope.
Capillary electrophoresis (CE) microchip has played an important role in DNA analysis in recent years. However, all CE microchips nowadays are builted by close channel. We have developed a novel capillary electrophoresis microchip by an open channel technique without channel walls. The open channel here is created by utilizing liquid dielectrophoresis (LDEP) principle to confine liquids above the LDEP electrodes and form a open channel around the vertical AC electric field at 100 kHz, 100 V. While we applied a parallel DC electric field, electroosmotic flow (EOF) was observed by FITC doping in buffer solution. The device has demonstrated injection, separation and detection of DNA between two parallel plates. The sample can be transported by electroosmotic flow at parallel DC electric field. DNA was preliminary analyzed at DC electric field with amplitude of 26.6 V/cm in an open channel micro CE chip by fluorescence microscope.
Page updated
Google Sites
Report abuse