This paper is divided into three sections. (1) a design of microfluidic chip; (2) the fabrication of microfluidic mold insert and microfluidic chip; (3) generation of microemulsion droplet. In the design of microfluidic chip section, the depth of microchannels were 50/200 μm respectively and CFD-RC simulation software was adopted to simulate the motion of fluids and emulsions in microfluidic channels. In the fabrication of microfluidic mold insert, we propose a new process of optical disc to manufacture microfluidic mold insert. This new process can prevent the damage on the mirror plate of the mold caused by the traditional process of optical disc. The mold system is composed of a mold insert (stamper) holder and a vacuum system, which is used to join the mold insert with the mold. In this way, the time to change the stamper is drastically decreased. Therefore, it can improve the utility rate of injection molding. In the microfluidic chips of injection molding, we investigate the influence of controlled factors on the quality properties (depth of replication rate, width deviation, birefringence, tilt and surface roughness) of microfluidic chips. Those controlled factors include mold temperature, cylinder temperature, clamping force and injection speed. In the microemulsion generation, we use cross-junction microchannel to form uniform water-in-oil (w/o) emulsions. We prove that the size of these emulsion drops can be easily controlled by adjusting the ratio of disperser phase flow / continue phase flow. These emulsion drops, consisting of 1.5% (w/v) sodium alginate (Na-alginate), are then dripped into a solution containing 20% (w/v) calcium chloride (CaCl2) to create Ca-alginate microcapsules in an efficient manner. We apply Taguchi method to investigate the influence of controlled factors on the size of microemulsion drops which include the flow rate of dispersed phase flow, flow rate ratio, viscosity and surfactant concentration.