This lipid bilayer formation system is based upon connecting aqueous volumes immersed in a hydrophobic phase containing lipids. Briefly, a lipid monolayer self-assembles at the water-oil boundary of the aqueous components, and when two components are brought into contact, a bilayer spontaneously forms between them. This formation system and method have several advantages. Full-oil-phase environment prevent aqueous droplet evaporation in oil chamber. To increase the lifetime of bilayer, hydrogel-supporting design makes bilayer strong. In experiments, the symmetric bilayer has been formed to discuss the factors that might afford stability and lifetime of bilayer. In bilayer formation experiment with optical image, the optical image was viewed by using inverted fluorescence microscopy, and formation success rate is 83%. The formation and lifetime of bilayer recorded in video clip through CCD. The longest lifetime of bilayer is 15 minutes. The electrophysiological recording measures by using patch clamp. The electrophysiological signal was detected through all formation timeline, and the longest lifetime of bilayer is about 1 minute. Bilayer capacitance per area is 0.32 μF/cm2.