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黃家聖 (Chia-Sheng Huang)
黃家聖 (Chia-Sheng Huang)
Microfabrication of Aligned Patterned Cells for Multisite Signal Recording of Live Cultured Neurons
Microfabrication of Aligned Patterned Cells for Multisite Signal Recording of Live Cultured Neurons
以微機械技術製造圖形式細胞 應用於活體神經訊號量測之研究
以微機械技術製造圖形式細胞 應用於活體神經訊號量測之研究
Manipulating neuron outgrowth and regeneration is important and attractive in the research fields of biomedical, neurological and tissue engineering. Over the last ten years, many bio-techniques such as μCP and MIMIC were developed on generating artificial micropatterns of extracellular matrix proteins (ECM) for guiding neuron to designed positions.
Manipulating neuron outgrowth and regeneration is important and attractive in the research fields of biomedical, neurological and tissue engineering. Over the last ten years, many bio-techniques such as μCP and MIMIC were developed on generating artificial micropatterns of extracellular matrix proteins (ECM) for guiding neuron to designed positions.
In this study, we propose a new scheme, i.e. open-top SU-8 microfluidic channel, to generate micropatterns of ECM proteins to guide and control axons outgrowth in preferred directions and at designed positions by confinement in a microfluidic system. The open-top SU-8 microfluidic channel requires only a small volume (~0.5μl) of laminin solution to fill the two tanks and microchannel.
In this study, we propose a new scheme, i.e. open-top SU-8 microfluidic channel, to generate micropatterns of ECM proteins to guide and control axons outgrowth in preferred directions and at designed positions by confinement in a microfluidic system. The open-top SU-8 microfluidic channel requires only a small volume (~0.5μl) of laminin solution to fill the two tanks and microchannel.
The culturing of retinal ganglion cells (RGCs) was performed using injured goldfish. The experimental results verify that the open-top SU-8 microfluidic channel is not only workable but also effective for neural guidance.
The culturing of retinal ganglion cells (RGCs) was performed using injured goldfish. The experimental results verify that the open-top SU-8 microfluidic channel is not only workable but also effective for neural guidance.
Besides, the micromachined open-top SU-8 microfluidic channel shows good integration ability of microelectronic device of microelectrode array (MEA) into the microfluidic channels, which solve the problem of transferring micro protein patterns on microelectronic device.
Besides, the micromachined open-top SU-8 microfluidic channel shows good integration ability of microelectronic device of microelectrode array (MEA) into the microfluidic channels, which solve the problem of transferring micro protein patterns on microelectronic device.
[1] C.-S. Huang, J. Chung, Y.-T. Cheng and W. Hsu (January 2007). Thermal Bimetallic Microactuators by Ni and Ni-diamond Nanocomposite, The 2nd Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, Bangkok, Thailand.
[2] C.-S. Huang, Y.-T. Cheng, C.-J. Yeh, H.-K. Liu, and W. Hsu (June 2009). Young’s modulus and fatigue lifetime improvements by diamond size effect on electroplated Ni-diamond nanocomposite, The 15th International Conference on Solid-State Sensors, Actuators and Microsystems, Denver, U.S.A..
[2] C.-S. Huang, Y.-T. Cheng, C.-J. Yeh, H.-K. Liu, and W. Hsu (June 2009). Young’s modulus and fatigue lifetime improvements by diamond size effect on electroplated Ni-diamond nanocomposite, The 15th International Conference on Solid-State Sensors, Actuators and Microsystems, Denver, U.S.A..
[3] C.-S. Huang, Y.-T. Cheng, J. Chung and W. Hsu, 2009, "Investigation of Ni-based Thermal Bimaterial Structure for Sensor and Actuator Application." Sensors and Actuators :A 2009, 149, 298-304.
[3] C.-S. Huang, Y.-T. Cheng, J. Chung and W. Hsu, 2009, "Investigation of Ni-based Thermal Bimaterial Structure for Sensor and Actuator Application." Sensors and Actuators :A 2009, 149, 298-304.
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