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胡皓宇 (Hao-Yu Hu)
胡皓宇 (Hao-Yu Hu)
Integration of Capillary Electrophoresis and Liquid Dielectrophoresis
Integration of Capillary Electrophoresis and Liquid Dielectrophoresis
整合液體介電泳及毛細管電泳晶片之研究
整合液體介電泳及毛細管電泳晶片之研究
In this study, we propose a capillary electrophoresis (CE) in a wall-less liquid dielectrophoresis (LDEP)-formed virtual microchannel. And further, two perpendicular electric fields are manipulated individually to effect the electroosmotic flow (EOF). The different charged particles and DNA are separated by utilizing the chip.
In this study, we propose a capillary electrophoresis (CE) in a wall-less liquid dielectrophoresis (LDEP)-formed virtual microchannel. And further, two perpendicular electric fields are manipulated individually to effect the electroosmotic flow (EOF). The different charged particles and DNA are separated by utilizing the chip.
When a 80 Vrms and 100 kHz signal was applied, the liquid containing 20 mM borax and 5 mM sodium dodecyl sulfate (SDS) was pumped into the virtual microchannel by following the electrode pattern on the bottom plate. The dimensions of the DEP-formed microchannel were 500 um in width, 3 cm in length, and 25 um in height. Then, a DC electric field is applied along the virtual channel to execute the electrophoresis process. The EOF at the velocity of 219.39 um/s is achieved when the applied DC electric field is 26.67 V/cm. Besides, while increasing the LDEP AC electric field with a DC bias between top and bottom plate, the EOF increased spontaneously. A digital cross-channel injection system is successfully demonstrated and integrated into the chip. By this system, the sample can be injected into the main channel and separated by CE process.
When a 80 Vrms and 100 kHz signal was applied, the liquid containing 20 mM borax and 5 mM sodium dodecyl sulfate (SDS) was pumped into the virtual microchannel by following the electrode pattern on the bottom plate. The dimensions of the DEP-formed microchannel were 500 um in width, 3 cm in length, and 25 um in height. Then, a DC electric field is applied along the virtual channel to execute the electrophoresis process. The EOF at the velocity of 219.39 um/s is achieved when the applied DC electric field is 26.67 V/cm. Besides, while increasing the LDEP AC electric field with a DC bias between top and bottom plate, the EOF increased spontaneously. A digital cross-channel injection system is successfully demonstrated and integrated into the chip. By this system, the sample can be injected into the main channel and separated by CE process.
[1] H.-Y. Hu, C.-Y. Huang, S.-K. Fan, and W. Hsu (October 2009). Electroosmotic Flow in Dielectrophoresis-Formed Microchannel, The 3rd IEEE International Conference on Nano/Molecular Medicine and Engineering, Tainan, Taiwan.
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