When a 80 Vrms and 100 kHz signal was applied, the liquid containing 20 mM borax and 5 mM sodium dodecyl sulfate (SDS) was pumped into the virtual microchannel by following the electrode pattern on the bottom plate. The dimensions of the DEP-formed microchannel were 500 um in width, 3 cm in length, and 25 um in height. Then, a DC electric field is applied along the virtual channel to execute the electrophoresis process. The EOF at the velocity of 219.39 um/s is achieved when the applied DC electric field is 26.67 V/cm. Besides, while increasing the LDEP AC electric field with a DC bias between top and bottom plate, the EOF increased spontaneously. A digital cross-channel injection system is successfully demonstrated and integrated into the chip. By this system, the sample can be injected into the main channel and separated by CE process.