The most literature of miniaturized column is designed to the circular pillar array in the current, and the majority of the material is silicon-based or polymer-based. Silicon-based column is the major direction of the research. The aspect ratio can be up to 40 and apply to high-performance liquid chromatography. The characterized of Polymer-based column can be made quickly by the reprographic technology, but often encounter the problem of inadequate Young's modulus will be fall. The research focus to make of high aspect ratio polymer-based column and test its separation efficiency. Hoping the aspect ratio of polymer column can be made as the same as silicon-based column, and shorten the separation time to achieve the purpose of rapid detection.