Microfluidic chips have been widely used in the biomedical analysis in recent years. In general, most microfluidic chips employ the physical channel wall to confine the liquid. Different from previous studies on physical channel wall, liquid dielectrophoresis-formed wall-less virtual microchannel is proposed in this study. Furthermore, a DC field was applied along the virtual microchannel to induce the capillary electrophoresis. These chips offer a number of advantages, such as simple configuration, absence of moving parts moving part, and programmable control. The geometry of virtual microchannel was determined by the electrode patterns on both top plate and bottom plate. Neutral fluorescent polystyrene beads are used to indicate the velocity of electroosmotic flow (EOF). When a 80 Vrms with 100 kHz signal was applied, the liquid containing 20 mM Borax buffer and 5 mM sodium dodecyl sulfate formed a virtual channel with 3 cm in length, 500 um in width, and 30 um in height in the medium of silicone oil. The effect of EOF velocity due to different voltages applied between two plates is investigated first. When the AC voltage was increased from 69 Vrms to 110 Vrms, the variation of EOF velocity was not obvious. When the DC bias voltage was increased from 5 V to 15 V, the EOF velocity increased from 18 um/s to 39 um/s. The effect of different voltages on the both ends of the virtual microchannel is also studied here. When the DC voltage was increased from 20 V to 40 V, the EOF velocity increased from 18 um/s to 39 um/s linearly.