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黃正鄴 (Cheng-Yeh Huang)
黃正鄴 (Cheng-Yeh Huang)
chengyeh.huang@gmail.com
Capillary Electrophoresis in Microfluidic Chips Based on Electrokinetic Forces
Capillary Electrophoresis in Microfluidic Chips Based on Electrokinetic Forces
應用電動力於毛細管電泳微流體晶片
應用電動力於毛細管電泳微流體晶片
Microfluidic chips have been widely used in the biomedical analysis in recent years. In general, most microfluidic chips employ the physical channel wall to confine the liquid. Different from previous studies on physical channel wall, liquid dielectrophoresis-formed wall-less virtual microchannel is proposed in this study. Furthermore, a DC field was applied along the virtual microchannel to induce the capillary electrophoresis. These chips offer a number of advantages, such as simple configuration, absence of moving parts moving part, and programmable control. The geometry of virtual microchannel was determined by the electrode patterns on both top plate and bottom plate. Neutral fluorescent polystyrene beads are used to indicate the velocity of electroosmotic flow (EOF). When a 80 Vrms with 100 kHz signal was applied, the liquid containing 20 mM Borax buffer and 5 mM sodium dodecyl sulfate formed a virtual channel with 3 cm in length, 500 um in width, and 30 um in height in the medium of silicone oil. The effect of EOF velocity due to different voltages applied between two plates is investigated first. When the AC voltage was increased from 69 Vrms to 110 Vrms, the variation of EOF velocity was not obvious. When the DC bias voltage was increased from 5 V to 15 V, the EOF velocity increased from 18 um/s to 39 um/s. The effect of different voltages on the both ends of the virtual microchannel is also studied here. When the DC voltage was increased from 20 V to 40 V, the EOF velocity increased from 18 um/s to 39 um/s linearly.
Microfluidic chips have been widely used in the biomedical analysis in recent years. In general, most microfluidic chips employ the physical channel wall to confine the liquid. Different from previous studies on physical channel wall, liquid dielectrophoresis-formed wall-less virtual microchannel is proposed in this study. Furthermore, a DC field was applied along the virtual microchannel to induce the capillary electrophoresis. These chips offer a number of advantages, such as simple configuration, absence of moving parts moving part, and programmable control. The geometry of virtual microchannel was determined by the electrode patterns on both top plate and bottom plate. Neutral fluorescent polystyrene beads are used to indicate the velocity of electroosmotic flow (EOF). When a 80 Vrms with 100 kHz signal was applied, the liquid containing 20 mM Borax buffer and 5 mM sodium dodecyl sulfate formed a virtual channel with 3 cm in length, 500 um in width, and 30 um in height in the medium of silicone oil. The effect of EOF velocity due to different voltages applied between two plates is investigated first. When the AC voltage was increased from 69 Vrms to 110 Vrms, the variation of EOF velocity was not obvious. When the DC bias voltage was increased from 5 V to 15 V, the EOF velocity increased from 18 um/s to 39 um/s. The effect of different voltages on the both ends of the virtual microchannel is also studied here. When the DC voltage was increased from 20 V to 40 V, the EOF velocity increased from 18 um/s to 39 um/s linearly.
International conference papers
1. C.-Y. Huang, S.-K. Fan, and W. Hsu, "Capillary electrophoresis in virtual microchannel based on dielectrophoresis," in The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011), Seattle, USA, September 2011.
1. C.-Y. Huang, S.-K. Fan, and W. Hsu, "Capillary electrophoresis in virtual microchannel based on dielectrophoresis," in The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011), Seattle, USA, September 2011.
2. C.-Y. Huang, S.-K. Fan, and W. Hsu, "Electroosmotic flow control in a virtual microchannel based on liquid dielectrophoresis" in The 6th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2011), Kaohsiung, Taiwan, February 2011. (Oral)
3. H.-Y. Hu, C.-Y. Huang, S.-K. Fan, and W. Hsu, "Electroosmotic flow in dielectrophoresis-formed microchannel," in The 3rd IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2009), Tainan, Taiwan, October 2009. (Oral)
3. H.-Y. Hu, C.-Y. Huang, S.-K. Fan, and W. Hsu, "Electroosmotic flow in dielectrophoresis-formed microchannel," in The 3rd IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2009), Tainan, Taiwan, October 2009. (Oral)
Domestic conference paper
Domestic conference paper
1. C.-Y. Huang, H.-Y. Hu, S.-K. Fan, and W. Hsu, "General digital microfluidic platform: capillary electrophoresis-based separation of micro-particle in electric-field-controlled virtual microchannel," in Taiwan Scientific Symposium, Birmingham, UK. (Invited talk)
1. C.-Y. Huang, H.-Y. Hu, S.-K. Fan, and W. Hsu, "General digital microfluidic platform: capillary electrophoresis-based separation of micro-particle in electric-field-controlled virtual microchannel," in Taiwan Scientific Symposium, Birmingham, UK. (Invited talk)
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