Abstract :
Vibrio campbellii is one of the leading causes of luminous vibriosis, especially in post larvae shrimp cultured in hatcheries. With the proper environment, this bacterium can form a complex structure known as a biofilm to attach to the surface of the environment. These situations led bacteria and their biofilm to become more resilience and difficult to remove. This research aimed to study the efficiency of lytic bacteriophages OPA17 and OTA22 at different multiplicities of infections (MOIs) for the inhibition and removal of V. campbellii PSU3280 biofilm. The results from this research show that phages OPA17 and OTA22 at the MOI 1 was the most effective in reducing the number of V. campbellii PSU3280. For the inhibition of biofilm, phages OPA17 and OTA22 at the highest MOI (10,000) was the most effective in biofilm inhibition when treated for 4 hours. For biofilm removal, the best efficiency was found in biofilm treated with phage OPA17 at MOI 1 for 4 hours. No differences were found in biofilm treated with phage OPA17 at different MOI and periods and the biofilm removal efficiency was independent of MOI and treated time. Similar results were found in phage OTA22, and the results were consistent with the results from the scanning electron microscope (SEM). In conclusion, both phages at the concentrations of MOI 1 were the most effective in reducing the planktonic V. cambellii PSU3280. Both phages exhibited good activity to inhibit or remove V. campbellii PSU3280 biofilm. However, further study about physiological characteristics of these phages is still needed for further applications.
Keywords : Vibrio campbellii, Bacteriophage, Biofilm removal