Hardy-Weinberg Class Activity (F1 Generation)

Activity A

Introduction

In this activity, your class will simulate a breeding population of diploid organisms, Wisconsin Fast Plants. You have four cards, which represent gametes produced during meiosis. The letter on the card represents an allele that is inherited with the gamete. You will contribute one gamete to each of your offspring. Everyone in the class will begin with the same four cards, two of ANL and two of anl.

Procedure

1. Place your cards (two anl cards and two ANL cards) face down and shuffle them until you do not know which card is which. Record the initial frequency of p and q (Recall that if 60% are of a specific type, that the frequency of that type is 0.60).

2. Stack your cards, continuing to keep them face down.

3. Pair with a classmate. Draw the top card from each stack. These cards represent the genotype of your first offspring. One of you should record this as Generation 1 Genotype in Table 1.

4. Each of you should take back your original card and reshuffle your stacks, keeping all of the cards face down.

5. Draw another card from each stack. These cards represent the genotype of your second offspring. The person who has not yet recorded a Generation 1 Genotype should now record this as the Generation 1 Genotype on their Table 1.

6. Now, assume the genotype of your recorded generation 1. This means that you should have four cards total, two matching each allele recorded in generation 1. For example, if you recorded anl/anl then you should have four anl cards now. Additional cards may be obtained from your instructor.

7. Randomly pair with another student and repeat this process for five generations, recording your results in Table 1 (Tab 3). Before each new mating, assume the genotype of the newly recorded generation.

8. Collect the class data for each generation and record it in Table 2.

9. Using the class data, determine the frequencies for each allele (anl or ANL) for each generation and record it in Table 3 (Tab 3). To determine frequency, divide the total number of an allele in a generation by the total number of alleles in that generation.

10. Answer the questions for Activity A.