過去の研究
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このページでは，とりあえず，論文や発表のリストを載せます（全部ではありません）．
そのうえで，可能な限り，どんな論文なのか，説明したいと思います．
Translational resistivity/conductivity of coding sequences during exponential growth of Esherichia coli. Takai, K. (Jan 12, 2017) J. Theor. Biol., 413, 66-71. doi: 10.1016/j.jtbi.2016.11.015
SfiNX: a method for assembly of protein coding sequences with high success rates. Takai, K.* and Hisamatsu, K. (May 2016). Biotechnol. Lett., 38(5), 773-778. doi: 10.1007/s10529-016-2042-2 First online Jan. 12, 2016.
--> The accepted manuscript is available here. Googleドライブのフォルダです．
CodHonEditor: Spreadsheets for Codon Optimization and Editing of Protein Coding Sequences. Takai, K. * (2016) Nucleosides Nucleotides Nucleic Acids, 35(5), 223-232. doi: 10.1080/15257770.2015.1127962
-> The accepted manuscript is available here. このリンク先はGoogle Driveのフォルダになっています．Manuscript本体は受理されたものそのままを（フォントの色を黒にして）PDFに変換したものです．7zファイルとodsファイルが付いていますが，7zファイルはExcel版，odsファイルはLibreOffice版のCodHonEditorです．ただし，LibreOffice版については，少し使いやすくしたものがありますので，順次公開したいと考えています．
Use of domain enzymes from wheat RNA ligase for in vitro preparation of RNA molecules.
Makino, S., Sawasaki, T., Endo, Y ., and Takai, K.* (2011)
Biochem. Biophys. Res. Commun., 404(4), 1050-1054.
Ribosome rescue by Escherichia coli ArfA (YhdL) in the absence of trans-translation system.
Chadani, Y., Ono, K., Ozawa, S., Takahashi, Y., Takai, K., Nanamiya, H., Tozawa, Y., Kutsukake, K., and Abo, T. (2010)
Mol. Microbiol., 78(4), 796-808.
In vitro dissection revealed that the kinase domain of wheat RNA ligase is physically isolatable from the flanking domains as a non-overlapping domain enzyme.
Makino, S., Sawasaki, T., Endo, Y., and Takai, K.*
Biochem. Biophys. Res. Commun., 397(4), 762-766 (Jul 8, 2010).
Modified uridines with C5-methylene substituents at the first position of the tRNA anticodon stabilize U·G wobble pairing during decoding.
Kurata, S., Weixlbaumer, A., Ohtsuki, T., Wada, T., Kirino, Y., Takai, K., Watanabe, K., Ramakrishnan, V., and Suzuki, T.*
J. Biol. Chem., 283(27), 18801-18811. Jul 4, 2008 (published online on May 2, 2008)
Covalent circularization of exogenous RNA during incubation with a wheat embryo cell extract.
Makino, S., Sawasaki, T., Tozawa, Y., Endo, Y., and Takai, K.*
Biochem. Biophys. Res. Commun., 347(4), 1080-1087 (2006). Sep 8, 2006.
Classification of the possible pairs between the first anticodon and the third codon positions based on a simple model assuming two geometries with which the pairing effectively potentiates the decoding complex.
Takai, K.
J. Theor. Biol., 242(3), 564-580 (2006). Oct 7, 2006.
Hirano, N., Sawasaki, T., Tozawa, Y., Endo, Y., and Takai, K.*
Proteins, 64(2), 343-354 (2006). Aug 1, 2006. doi: 10.1002/prot.21008
[PubMed]
Selection of 5'-untranslated sequences that enhance initiation of translation in a cell-free protein synthesis from wheat embryos.
Kamura, N., Sawasaki, T., Kasahara, Y., Takai, K., and Endo, Y.*
Bioorg. Med. Chem. Lett., 15(24), 5402-5406 (2005). Dec 15, 2005.
Formation of circular polysomes in wheat germ cell-free protein synthesis system.
Mardin, K., Sawasaki, T., Kamura, N., Takai, K., Ogasawara, T., Yazaki, K., Takei, T., Miura, K.-I., and Endo, Y.*
FEBS Lett., 562(1-3), 155-159 (2004). March 26, 2004.
RALyase; a terminator of elongation function of depurinated ribosomes.
Ozawa, A., Sawasaki, T., Takai, K., Uchiumi, T., Hori, H., and Endo Y.*
FEBS Lett., 555(3), 455-458 (2003). Dec 18, 2003.
Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ.
Kawasaki, T., Gouda, M. D., Sawasaki, T., Takai, K., and Endo, Y.*
Eur. J. Biochem., 270(23), 4780-4786 (2003). Dec., 2003.
Roles of 5-substituents of tRNA wobble uridines in the recognition of purine-ending codons.
Takai, K.* and Yokoyama, S.
Nucleic Acids Res., 31(22), 6383-6391 (2003). Nov. 15, 2003. doi: 10.1093/nar/gkg839
[PubMed]