jzs.10003

Determination of tetracycline hydrochloride in some pharmaceutical products using flow injection spectrophotometric techniquel products using flow injection spectrophotometric technique

Shirwan Omer baban 1 Rasul Jameel Ali 2

1 Chemistry Department, College of Education Science, Salahaddin University-Hawler, Iraqi-Kurdistan Region 2 Ministry of

Agriculture and Water Resources, Erbil

2 Clinical Biochemistry department, College of Health Sciences, Hawler Medical University, Hawler / Iraqi-Kurdistan Region

E- shirwan_baban@yahoo.co.uk

Article info

Original: 17 April. 2015

Revised:11 August. 2015

Accepted:30 August.  2015

Published online: 20 March.  2016

Key Words:

Tetracycline

hydrochloride, 2-

aminopyridine, flow

injection

spectrophotometric

Abstract

A simple, rapid, and sensitive flow injection spectrophotometric system for

determination of tetracycline hydrochloride (TCH) in pharmaceutical products. The

method is based on diazotization of 2-aminopyridine and subsequent coupling with

tetracycline hydrochloride in alkaline medium. The formed azo-dye showed maximum

absorbance at 388 nm and the calibration curve in the linear range of 25.0-150.0 μg/ml

of tetracycline hydrochloride with correlation coefficient 0.9988, while the detection

limit was 10 μg/ml and sample frequency 123 samples/hour. The accuracy and

precision data for determination of TCH by flow injection analysis method for triplicate

injection at three concentration levels(25, 80 and 150 μg/ml) the relative standard

deviation (RSD %) are 1.04, 0.247 and 1.75%, and relative error percent (Erel) are -

3.92, +1.06 and -1.9%. The proposed method was successfully applied to the

determination of tetracycline hydrochloride in pharmaceutical products. The results

obtained were in good agreement with those obtained by reference method.