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Hoshyar A. Azeez, Ahmad H. Ameen and Shewa A. Faqe
Biology Dept., College of Science, Sulaimani University, Bakrajo street, Sulaimaniyah-Iraq,
Filed Crop Dept., Faculty of Agricultural Science, Sulamani University, Bakrajo campus- Awall Street, Sulaimaniyah- Iraq
DOI: https://doi.org/10.17656/jzs.10608
Original:24 August 2016, Revised: 28 December 2016, Accepted: 9 January 2017, Published online: 20 June 2017
Abstract
In this research work some bioactive molecules of Hypericum triqutrifolium Turra., were evaluated at vegetative (V), flowering (Fl) and fruiting (Fr)} developing stages during the growing seasons as natural products. HPLC was used for qualitative and quantitative of bioactive compounds, which compared with expected compounds such as: Sirginic, Querctrin, Apeginin, Karmeferol, Hypericin and Hyperforin. Different diversity and phytochemical composition among (V), (Fl), and (Fr) were observed. The highest concentration of quercitrin was obtained when plants fruited, whereas apigenin, kameferol and sriginic acid showed highest levels during flower development, while hyperforin in fruiting stage accumulated the highest concentration compared to vegetative and flowering. On the other hand, vegetative stage accumulated larger amounts of hypericin. Regarding to the bioactive accumulation in the individual plant parts, fresh fruit accumulate the highest level of hyperforin at fruiting stage, while it was not detected in stem during flowering and fruiting, whereas leaves produce more hypericin at flowering time
Key Words:
Hypericumtriquetrifolium,
Phenolic compoundsHyperforin,
Hypercicn, Plant development.
References
[1] Balandrin, M.J. and J.A. Klocke. "Medicinal, aromatic and industrial materials from plants. In Y.P.S". Bajaj (ed.), Biotechnology in Agriculture and Forestry. Medicinal and Aromatic Plant, Vol. 4. Springer-Verlag, Berlin, Heidelberg, pp. 1-36, (1988).
[2] Dias A.C.P., Seabra R.M., Andrade P.B., Ferreres F. and Fernandes-Ferreria M. "Xanthone biosynthesis and accumulation in calli and suspended cells of Hypericumandrosaemum". Plant Sci. Vol. 150, pp. 93 – 101, (2000).
[3] Patocka J. "The chemistry, pharmacology, and toxicology of the biologically active constituents of the herb HypericumperforatumL". J. Appl. Biomed.Vol. 1, pp. 61– 73, (2003).
[4] Rahimi, R,and Kiani, S. "Chemical compound and Therapeutic effects of Hypericumperforatum". Der Pharmacia Lettre, Vol. 8, No. 9, pp. 237-241, (2016).
[5] Isfahlan A. J., Mahmoodzadeh, A. ,Hasanzadeh, A. "Antioxidant and antiradical activities of phenolic extracts from Iranian almond (Prunusamygdalus L.) hullsand shells". Turk J. Biol. Vol. 34, pp. 165-173, (2010).
[6] Chu YH, Chang CL, Hsu HF. "Flavonoid content of several vegetables and their antioxidant activity". J Sci Food Agric. Vol. 80, pp. 561-566, (2000).
[7] GhosianMoghaddam, M. H., Roghani, M., and Malek, M. "Effect of Hypericumperforatum aqueous extracts on serum lipids, Aminotransferases, and Lipid Peroxidation in Hyperlipidemic Rats". ResCardiovasc Med. Vol. 5, No. 2, e31326, (2016).
[8] Gastpar, M, and Zeller K. "Hypericum-extrakt STW3 und sertralinzurbehandlung der mittelschweren depression". Psychopharmakotherapie Vol. 12, pp. 146-153, (2005).
[9] Cirak, C., Radusiene, J., Janulis, V. and Ivanauskas, L. "Secondary metabolites in Hypericumperforatum: variation among plant parts and phonological stages". Bot. Helv., Vol. 117, pp. 29-36, (2007).
[10] Hosni, K., Msaada, K., Taarit, M.B. and Marzouk, B. "Phonological variations of secondary metabolites from HypericumtriquetrifoliumTurra". Biochemical Sys.and Ecol. Vol. 39, pp. 43-50, (2011).
[11] Ghavamaldin, A., Aptin, R., Khalil, P., Mansour, G. and Mariamalsadat, T. "Study of variation of biochemical components in Hypericum perforatum L. growm in north of Iran". J. of Medicinal Plants Res., Vol. 6, No. 3, pp. 366-372, (2012).
[12] Azeez, H. A., and Ibrahim K. M. "Hypericum Triquetrifolium Callus Cultures a Potential Source of Phenolics and Flavonoids". Journal of Zankoy Sulaimani- Part A, Special Issue, Vol. 16, (2014).
[13] Azeez, H. A., and Ibrahim K. M. "Effect of Biotic Elicitors on Secondary Metabolite Production in Cell Suspensions of Hypericum triquetrifolium Turra". Bulletin UASVM Horticulture,.Print ISSN 1843-5254; Electronic ISSN 1843-5394, Vol 70, No. 1, pp. 26-33, (2013).
[14] Benkiki, N., Kabouche, Z., Tillequin, F., Verite, P., Chosson, E. & Seguin, E. "A new polyisoprenylatedphloroglucinolderivativefromHypericumperfoliatum (Clusiaceae)". Z Naturforsch Vol. 58, pp. 655–658, (2003).
[15] Couladis,M.,Baziou,P., Petrakis,P.V., Harvala, C. "Essential oil composition of HypericumperfoliatumL". growing in different locations in Greece, Flavour Fragrance J. Vol. 16, pp. 204-206, (2001).
[16] EMA/HMPC. "Community Herbal Monograph on HypericumperforatumL., Herba (Well-Established Medicinal Use)". Available online: http://www.ema.europa.eu/docs/ en_GB/document_library/Herbal-Community_herbal_monograph/2010/01/WC500059145,(2013).
[17] "European Directorate for the Quality of Medicines & Health Care". European Pharmacopoea, 6th ed.; Council of Europe: Strasbourgh, France; Vol. 2, pp. 2958–2959, (2007).
[18] Tortoriello, J., Zamilpa, A., Herrera, A., Romero-Cerecero, O., and Mental, S. "Determination of Hypericin and Hyperforin content in different phytopharmaceuticals and dietary supplements containing Hypericumperforatum extract". Salud Mental,. Vol. 26, No. 4, pp. 59-63, (2003).
[19] Duncan, D.B. "Multiple Range and Multiple F-test", Biometrics, Vol. 11, pp. 1-42, (1995).
[20] Cirak, C., Saglam, B., Ayan, A.K. and Kevseroglu, K. "Morphogentic and diurnal variation of hypericin in some Hypericum species from Turkey during the course of ontogenesis", Biochem. Syst. Ecol., Vol. 34, pp. 1-13, (2006).
[21] Tekel’ova, D., Repcak, M., Zemkova, E. and Toth, J. "Quantitative changes of dianthrones, hyperforin and flavonoids content in the flower ontogenesis of Hypericumperforatum". Planta Med., Vol. 66, pp. 778−780, (2000).
[22] Sirvent, T.M., Krasnoff, S.B. and Gibson, D.M. "Induction of hypericins and hyperforins in Hypericumperforatum in response to damage by herbivores". J. Chem. Ecol. Vol. 29, pp. 2667-2681, (2003).
[23] Beerhues, L. "Hyperforin. Phytochemistry", Vol. 67, pp. 2201–2207, (2006).
[24] Soelberg, J., Jørgensen, L. B.andJäger, A. K. "Hyperforin accumulates in the translucent glands of Hypericumperforatum". Ann Bot. Vol. 99, pp. 1097−1100, (2007).
[25] Holscher, D., Shroff, R., Knop, K., Gottschaldt, M., Crecelius, A., Schneider, B.,Heckel, D. G, Schubert, U.S.andSvatos, A. "Matrix-free UV-laser desorption/ionization (LDI) mass spectrometric imaging at the single-cell level: distribution of secondary metabolites in Arabidopsis thaliana and Hypericum species". Plant J Vol. 60, pp. 907−918, (2009).
[26] Briskin, D.P. and Gawienowski, M. C. "Differential effects of light and nitrogen on production of hypericins and leaf glands in Hypericumperforatum". Plant Physiol. Biochem., Vol. 39, pp. 1075−1081, (2001).
[27] Cirak, C., Radusiene, L. and Arslan, B. "Variation of bioactive substances in Hypericummontbetii during plant growth". Nat. Prod. Res., Vol. 22, pp. 246- 252, (2008).
[28] Piovan, A., Filippini, R., Caniato, R., Borsarini, A., Maleci., L.B. and Cappelletti, EM. "Detection of hypericin in the “red glands” of Hypericumelodes by ESI-MS/MS". Phytochemistry, Vol. 65, pp. 411-414, (2004).
[29] Cirak, C., Saglam, B., Ayan, A.K. and Kevseroglu, K. "Morphogentic and diurnal variation of hypericin in some Hypericum species from Turkey during the course of ontogenesis", Biochem. Syst. Ecol., Vol. 34, pp. 1-13, (2006).
[30] Ayan, A.K. and Cirak, C. "Variation of hypericin in HypericumtriquetrifoliumTurra. growing in different locations of Turkey during plant growth". Nat. Prod. Res., Vol. 22, pp. 1597-1604, (2008b).
[31] Kirakosyan, A., Vardapetyan, H. and Charchoglyan, A. "The content of hypericin and pseudohypericin in cell culture of HypericumperforatumL". Russian J. Plant Physiol., Vol. 47, pp. 301-306, (2000).
[32] Ciccarelli, D, Andreucci AC &PagniAM. "Translucent glands and secretory canals in Hypericumperforatum L. (Hypericaceae): morphological, anatomical and histochemical studies during the course of ontogenesis". Ann Bot Vol. 88, pp. 637−644, (2001).
[33] De-Smet, P. and Nolen, W.A. "St. John's wort as an antidepressant". British Med. J., Vol. 313, pp. 241-242, (1996).
[34] Cirak, C., Radusiene, J., Janulis, V., and Ivanauskas. "Pseudohypericin and Hyperforin in Hypericumperforatum from Northern Turkey: Variation among Populations, Plant Partsand Phenological Stages". Journal of integrative plant biology,Vol. 50, Issue 5. pp. 575–580, (2008).
[35] Cirak, C., Radusiene, J., Janulis, V. and Ivanauskas, L. "Secondary metabolites in Hypericumperforatum: variation among plant parts and phonological stages". Bot. Helv., Vol. 117, pp. 29-36, (2007).
[36] Ayan, A.k., and Cirak, C. "Hypericin and psudohypericin contents in some Hypericum species growing in Turkey". Pharmaceutical Biology, Vol. 46, No. 4, pp. 288–291, (2008 a).
[37] Radušienė, J., Bagdonaite, E., and Kazlauskas, S. "Morphological and chemical evaluation on Hypericumperforatum and H. maculatum in Lithuania". ActaHort (ISHS) Vol. 629, pp. 55-62, (2004).
[38] Cirak, C., Saglam, B., Ayan, AK., and Kevseroglu, K. "Morphogenetic and diurnal variation of hypericin in some Hypericum species from Turkey during the course of ontogenesiss". Biochem. Syst. Ecol. Vol. 34, No. 1, pp. 1-13, (2006).
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