jzs-10793

Biochemical and molecular identification with antimicrobial susceptibility of bacterial species isolated from organs and tissues of Alectoris chukar subspecies Kurdistanica

Heshu Sulaiman Rahman1,2*, Kwestan Najm Ali3, Nawzad Rashid Abdulrahman3, Peshnyar Muhammad Atta Rashid4, Alan Ahmed Mahmood2, Hemn Hassan Othman5, Kawa Amin1, Rasedee Abdullah6

1College of Medicine, University of Sulaimani, Sulaimani, Kurdistan Region- Iraq

2College of Health Sciences, Komar University of Science and Technology, Chaq-Chaq Qularaesee, Sarchinar District, Sulaimani, Kurdistan Region- Iraq

3College of Veterinary Medicine, University of Sulaimani, Sulaimani, Kurdistan Region- Iraq

 4Molecular Diagnostic Laboratory, Sulaimani Veterinary Directorate, Sulaimani, Kurdistan Region- Iraq

5College of Pharmacy, University of Sulaimani, Kurdistan Region- Iraq

6Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Original: 20 January 2020       Revised: 10 March 2020     Accepted: 5 April 2020        Published online: 20 June 2020  

Doi Link; https://doi.org/10.17656/jzs.10793

Abstract

The current study was conducted on 50 Alectoris chukar subspecies Kurdestanica that was collected from Sulaymaniyah Province, Kurdistan Region, Northern Iraq, during the period of April to the end of September 2016. Samples of liver, gallbladder, spleen, kidneys, heart, lungs, gizzard, breast, and thigh muscle tissues were tested for bacterial isolates. Preliminary characterization of the isolated bacteria was carried out by morphological and biochemical methods. The VITEK 2® system was used to confirm the isolated species, while the polymerase chain reaction (PCR) was used for the detection of the resistance gene in the bacterial isolates. As a result, tested samples showed the presence of Staphylococcus sciuri and Escherichia coli. Additionally, antimicrobial susceptibility test was done to determine the bacterial susceptibility to various antibiotics and as a result; E. coli showed 100% susceptibility to penicillin, azithromycin, tetracycline, and doxycycline and 75% susceptibility to streptomycin. On the other hand, S. sciuri exhibited 75% susceptibility to azithromycin, penicillin, and doxycycline, 50% susceptibility to streptomycin, and 25% susceptibility to tetracycline. Molecular identification showed that only the S. sciuri isolates carried the methicillin-resistant mecA gene. To our knowledge, this is the first record of isolationof the S. sciuri methicillin-resistant mecA gene from A. chukar subspecies Kurdestanica.

Key Words: Bacterial isolates, wild bird, biochemical test, VITEK 2 system, antibiotic susceptibility, gene sequencing

 References

[1] Kline CG. "How to make money raising chukar partridge?" NCR, Inc., Eagle, San Diego, United States, (2002).

[2] Barbanera F, Guerrini M, Khan AA, Panayides P, Hadjigerou P, Sokos C, et al. "Human-mediated introgression of exotic chukar (Alectoris chukar, Galliformes) genes from East Asia into native Mediterranean partridges". Biological Invasions. Vol. 11, No. 2, pp. 333-348. (2009).

[3] Christensen GC. "The chukar partridge: its introduction, life history, and management". Biological Bulletin No. 4, Nevada Department of Fish and Game, USA, (1970).

[4] Ratti JT, Giudice JH. "Assessment of chukar and gray partridge populations and habitat in Hells Canyon", Department of Fish and Wildlife Resources, University of Idahom, Moscow, Russia, (2001).

[5] Barilani M, Bernard-Laurent A, Mucci N, Tabarroni C, Kark S, Garrido JAP, et al. "Hybridisation with introduced chukars (Alectoris chukar) threatens the gene pool integrity of native rock (A. graeca) and red-legged (A. rufa) partridge populations". Biological Conservation. Vol. 137, No. 1, pp. 57-69. (2007).

[6] Lahony SR, Al-Rawy MA. "New subspecies of chukar partridge Alectoris chukar (Gray 1830) (Phasianidae, Galliformes) from North East of Iraq with biological observations". Bulletin of the Iraq Natural History Museum. Vol. 11, No. 1, pp. 57-67. (2010).

[7] Hawramany SRO. "Ecology, behavior, reproduction, and classification of Alectoris chukar (Gray) with a comparison between sub-species found in Iraq". Master Thesis, College of Natural History Research Centre and Museum, University of Baghdad, Baghdad, Iraq, (2007).

[8] Zhala OIK, Shamall MAA. "Study on the parasites of chukar partridge Alectoris chukar from Shaqlawa district, Kurdistan region, Iraq". Tikrit Journal of Pure Science. Vol. 18, No. 3, pp. 26-30. (2012).

[9] Kloos WE. "Natural populations of the genus Staphylococcus". Annual Reviews of Microbiology. Vol.  34, pp. 559-592. (1980).

[10] Marsou R, Bes M, Boudouma M, Brun Y, Meugnier H, Freney J, Vandenesch F, Etienne J. Distribution of Staphylococcus sciuri subspecies among human clinical specimens, and profile of antibiotic resistance. Research in Microbiology. 1990; 150:531-541.

[11] Stepanovic S, Dakic I, Djukic S, Lozuk B, Svabic-Vlahovic M. Surgical wound infection associated with Staphylococcus sciuri. Scandinavian Journal of Infectious Diseases. 2002; 34:685-686.

[12] Dale E, Brown C. "Zoonotic diseases from poultry". Brazilian Journal of Veterinary Pathology. Vol. 6, No. 2, pp. 76-82. (2017).

[13] Allen HK, Donato J, Wang HH, Cloud-Hansen KA, Davies J, Handelsman J. "Call of the wild: antibiotic resistance genes in natural environments". Nature Reviews Microbiology. Vol. 8, No. 4, pp. 251-259. (2010).

[14] Stepanovic S, Ježek P, Vukovic D, Dakic I, Petráš P. "Isolation of members of the Staphylococcus sciuri group from urine and their relationship to urinary tract infections". Journal of Clinical Microbiology. Vol. 41, No. 11, pp. 5262-5264. (2003).

[15] Ozek K. "Protein ve enerji seviyeleri farklı rasyonların kapalı sartlar altında yetistirilen etlik kınalı kekliklerin (A. chukar) performansı, karkas, karkas karakterleri ve bazı kan parametrelerine etkileri". Sel Üni Fen Bil Enst Master Thesis, Konya. (2001).

[16] Erol İ. "Listeria monocytogenes, in Gıda Hijyeni ve Tek-nolojisi", 1s edition, Pozitif matbaacılık, Anakara, pp: 126- 134. (2007).

[17] Chen S, Wang Y, Chen F, Yang H, Gan M, Zheng SJ. "A highly pathogenic strain of Staphylococcus sciuri caused fatal exudative epidermitis in piglets". PLoS One. Vol. 2, No. 1, e147. (2007).

[18] Bam F, Hunt J, Abeyta C, Tran T. "FDA Bacteriological Analytical Manual". AOAC International, Gaithersburg, MD. Vol. 7, pp. 23. (1998).

[19] Johnson JG, Kunz LJ, Barron W, Ewing W. "Biochemical differentiation of the Enterobacteriaceae with the aid of lysine-iron-agar". Applied Microbiology. Vol. 14, No. 2, pp. 212-217. (1966).

[20] Carter GR and Cole JR. "Diagnostic procedure in veterinary bacteriology and mycology". Academic Press. (2012).

[21] Pincus DH. "Microbial identification using the bioMerieux VITEK® 2 System". Encyclopedia of Rapid Microbiological Methods. Vol. 1, pp. 1-32. (2006).

[22] Foster AW. "Rapid identification of microbes in positive blood cultures by matrix-assisted laser desorption/ionisation time-of-flight (Maldi-Tof) mass spectrometry-(Vitek MS—bioMérieux)". Journal of Clinical Microbiology. Vol. 51, No. 11, pp. 3717-3719. (2013).

[23] Nonhoff C, Rottiers S, Struelens MJ. "Evaluation of the Vitek 2 system for identification and antimicrobial susceptibility testing of Staphylococcus spp". Clinical Microbiology and Infection. Vol. 11, No. 2, pp. 150-153. (2005).

[24] Gentilini E, Denamiel G, Llorente P, Godaly S, Rebuelto M, DeGregorio O. "Antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Argentina". Journal of Dairy Science. Vol. 83, No. 6, pp. 1224-1227. (2000).

[25] Couto I, Sanches IS, Sá-Leão R, de Lencastre H. "Molecular characterization of Staphylococcus sciuri strains isolated from humans". Journal of Clinical Microbiology. Vol. 38, No. 3, pp. 1136-1143. (2000).

[26] Greisen K, Loeffelholz M, Purohit A, Leong D. "PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid". Journal of Clinical Microbiology. Vol. 32, pp. 335-351. (1994).

[27] Kozačinski L, Hadžiosmanović M, Zdolec N. "Microbiological quality of poultry meat on the Croatian market". Veterinarski Arhiv. Vol. 76, No. 4, pp. 305-313. (2006).

[28] Benskin CM, Wilson K, Jones K, Hartley IR. "Bacterial pathogens in wild birds: a review of the frequency and effects of infection". Biological Reviews. Vol. 84, No. 3, pp. 349-373. (2009).

[29] Jain A, Agarwal J, Bansal S. "Prevalence of methicillin-resistant, coagulase-negative staphylococci in neonatal intensive care units: findings from a tertiary care hospital in India". Journal of Medical Microbiology. Vol. 53, pp. 941-944. (2004).

[30] Dakić I, Morrison D, Vuković D, Savić B, Shittu A, Ježek P, et al. "Isolation and molecular characterization of Staphylococcus sciuri in the hospital environment". Journal of Clinical Microbiology. Vol. 43, No. 6, pp. 278-285. (2005).

[31] Shittu A, Lin J, Morrison D, Kolawole D. "Isolation and molecular characterization of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus associated with skin and soft-tissue infections". Journal of Medical Microbiology. Vol. 53, No. 1, pp. 51-55. (2004).

[32] Horii T, Suzuki Y, Kimura T, Kanno T, Maekawa M. "Intravenous catheter-related septic shock caused by Staphylococcus sciuri and Escherichia vulneris". Scandinavian Journal of Infectious Diseases. Vol.  33, No. 12, pp. 930-932. (2001).

[33] Wallet F, Stuit L, Boulanger E, Roussel-Delvallez M, Dequiedt P, Courcol RJ. "Peritonitis due to Staphylococcus sciuri in a patient on continuous ambulatory peritoneal dialysis". Scandinavian Journal of Infectious Diseases. Vol. 32, No. 6, pp. 697-698. (2000).

[34] Couto I, de Lencastre H, Severina E, Kloos W, Webster JA, Hubner RJ, et al. "Ubiquitous presence of a mecA homologue in natural isolates of Staphylococcus sciuri". Microbiology Drug Resistance. Vol. 2, pp. 377-391. (1996).

[35] Nemeghaire S, Argudín MA, Haesebrouck F, Butaye P. "Molecular epidemiology of methicillin-resistant Staphylococcus sciuri in healthy chickens". Veterinary Microbiology. Vol. 171, No. 3-4, pp. 357-363. (2014).

[36] Daki I, Morrison D, Vukovi D, Savi B, Shittu A, Jeek P, Hauschild T, Stepanovi S. "Isolation and molecular characterization of Staphylococcus sciuri in the hospital environment". Journal of Clinical Microbiology. Vol. 43, No. 6, pp. 2782-2785. (2005).

[37] Stepanović S, Dakić I, Djukić S, Lozuk B, Svabić-Vlahović M. "Surgical wound infection associated with Staphylococcus sciuri". Scandinavian Journal of Infectious Diseases. Vol. 34, No. 9, pp. 685-686. (2002).

[38] Nemeghaire S, Argudín MA, Feßler AT, Hauschild T, Schwarz S, Butaye P. "The ecological importance of the Staphylococcus sciuri species group as a reservoir for resistance and virulence genes". Veterinary Microbiology. Vol. 171, No. 3-4, pp. 342-356. (2014).

[39] Akbar A, Sitara U, Ali I, Khan MI, Phadungchob T, Anal AK. "Presence of Escherichia coli in poultry meat: a potential food safety threat". International Food Research Journal. Vol. 21, No. 3, pp. 941-945. (2014).

[40] Hossain MT, Siddique MP, Hossain FMA, Zinnah MA, Hossain MM, Alam MK, Rahman MT, Choudhury KA. "Isolation, identification, toxin profile and antibiogram of Escherichia coli isolated from broilers and layers in Mymensingh district of Bangladesh". Bangladesh Journal of Veterinary Medicines. Vol. 6, No. 1, pp. 01-05. (2008).

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