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1 Yousif M. S. N. Al-Barzinji, 2 Muqdad K. Ali
1 Dept. of Animal Resource, College of Agriculture, University of Salahaddin, 2 Dept. of Animal Production, Faculty of Agricultural Science, University of Sulamania
DOI: https://doi.org/10.17656/jzs.10326
Abstract:
RAPD-DNA markers were used to study genetic characterization among four
locations for Karadi sheep breed in Sulamania governorate. A total of 40 samples were
typed using twenty RAPD primers. Nine out of the twenty primers had clear bands,
which used to investigate the genetic variations among four locations(Halabjai Taza,
Penjwen, Halabja and Sharbazer) of same breed. Out of the nine primers 8 of them are
polymorphisms. A total of 119 bands were scored, of which 27 bands (25.05%) were
polymorphic and seven of polymorphic band were unique bands. For all location, Nei’s
gene diversity, Shannon index and percentage of polymorphic loci are respectively
averaged of 0.2361, 0.3415 and 34.60. Using unweighted pair-group method with
arithmetic average (UPGMA) dendrogram, the three clusters, the 1st cluster branch
consisted of the Karadi sheep in location 1 and 3, the 2nd cluster was including Karadi
sheep from location 2 and the 3rd one including sheep from location 4. These results
indicated that the Karadi sheep in location 1 and 3 is most genetically distant from the
Karadi sheep in location 2 .The dendrograms show that there are moderately genetic
diversity among Karadi sheep breed, were ranged from 0.251to 0.541. Based on the high
degree of genetic distance among the four locations it is concluded that there are widely
area for selection in this breed of sheep.
Keyword: Karadi sheep breed, Genetic distance, RAPD-PCR.
References
[1] FAO, Food and Agricultural Organization, FAO Yearbook. Production, 49, (1996).
[2] M. M. Al-Alaq, Y. Al-Fahad, T. Abass, T., The Statistical Atlas Agricultural Roadmap for
Agricultural Development (Green Economy) the Ministry of Planning (Central
organization of Statistics) and Agriculture, Iraq. (2011), (Arabic Reference).
[3] A. A. Al-Rawi, A. H. Al-Haboby, A. Salman, Small Ruminants Breeding, Reproduction
Physiology Research as Technology Transfer I Iraq. (W. Mourani, N. Haddad, Ed),
ICARDA West Asia Regional Program, Amman, Jordan. (1996).
[4] C. Diez-Tascon, R. P. Littlejohn, P. A. Almaeida, A. M. Crawford, Genetic variation
within the Merino sheep breed: analysis of closely related populations using
microsatellites. Anim. Genet. 31, pp. 243-251, (2000).
[5] J. F. Maddox, K. P. Davies, A. M. Crawford, D. J. Hulme, D. Vaiman, E. P. Cribiu, et al.,
An enhanced linkage map of the sheep genome comprising more than 1000 loci.
Genome Res. 11, pp. 1275–1289, (2001).
[6] R. Baumung, H. Simianer, I. Hoffmann, Genetic diversity studies in farm animals – a
survey. J. Anim. Breed. Genet. 121, pp. 361–373, (2004).
[7] E. V. Marle-Koster, L. H. Nel, Genetic markers and their application in livestock
breeding in South Africa. South Africa J. Anim. Sci. 33, pp. 1-10, (2003) .
[8] M. Raymond, F. Rousset, GENEPOP (version 3.3): Population genetics software for exact
tests and ecumenicism. J. Heredity. 86, pp. 248-249, (1995).
[9] E. R. Mahfouz, O. E. Othman, S. M. El Nahas, M. A. A. El Barody, Genetic Variation
between Some Egyptian Sheep Breeds Using RAPD-PCR. Res. J. of Cell and Mol.
Biol. 2 (2), pp. 46-52, ( 2008).
[10] M. M. Tariq, M. A. Bajwa, K. Jawasreh, M. A. Awan, F. Abbas, A. Waheed, et al.,
Characterization of four indigenous sheep breeds of Baluchistan, Pakistan by random
amplified polymorphic DNAs. African J. Biotech. 11(10), pp. 2581-2586, ( 2012) .
[11] H. El Hentati, M. Ben Hamouda, A. Chriki, Genetic diversity of two Tunisian sheep
breeds using random amplified polymorphic DNA (RAPD) analysis. African J.
Biotech. 11(17), pp. 4109-4115,( 2012).
[12] A. Okumus, L. Mercan, Genetic variation at karayaka sheep Herds based on random
amplified polymorphic DNA markers. Biotech. 6(4), pp. 543-548, (2007).
[13] V. Kumar, Random amplified polymorphic DNA analysis of four breeds of sheep in
Malaysia. M.Sc. thesis, Faculty of Vet. Med. and Anim. Sci., University of Putra,
Malaysia. (1999).
[14] S. R. Paiva, V. C. Silverio, A. A. Egito, C. McManus, D. A. de Feria, A. S. Mariante, et
al., Genetic variability of Brazilian hair sheep breeds. Pesq. agropec. bras, Brasilia.,
40(9), pp. 887-893, ( 2005).
[15] S. Kumar, A. P. Kolte, B. R. Yadav, S. Kumar, A. L. Arora, V. K. Singh, Genetic
variability among sheep breeds by random amplified polymorphic DNA-PCR.
Iranian J. Biotech. 7(4), pp. 482-486, ( 2008).
[16] M. Qasim, H. Ahmad, S. Ghafoor, S. G. Afridi, I. Muhammad, A. K. Imtiaz, Estimation
of Genetic Diversity in Sheep (Ovis aries) using Randomly Amplified Polymorphic
DNA. International J. of Anim. and Vet. Advances. 3(1), pp. 6-9, ( 2010).
[17] D. Aytekin, S. Boztepe, Determination of Genetic Polymorphism within Guney Karaman
Sheep-Breed via RAPD-PCR Method. issd_science_book. pp. 183-190, (2010).
[18] K. I. Jawasreh, I. M. Al-Rawashdeh, A. Al-Majali, H. Talafha, A. Eljarah, F. Awawdeh,
Genetic relatedness among Jordanian local Awassi lines Baladi, Sagri,and Blackface
and the black Najdi breed using RAPD analysis. Genomics and Quantitative Genet.
2, pp. 31-36, (2011).
[19] W. T. Cushwa, K. G. Dodds, A. M. Crawford, J. F. Medrano, Identification and genetic
mapping of random amplified polimorphic DNA (RAPD) markers to the sheep
genome. Mammalian Genome, 7, pp. 580–585, (1996).
[20] Z. B. Khaldi, B. Rekik, L. Haddad, S. S. Zourgui, Genetic characterization of three
ovine breeds in Tunisia using randomly amplified polymorphic DNA markers.
Livest. Res. Rural Dev., 22,(2010).
[21] S. R. Hlophe, Genetic variation between and within six selected South African sheep
breeds using random amplified polymorphic DNA and protein markers. MSc. Thesis.
Faculty of sci. and Agri. University of zululand, South Africa. (2011).
[22] B. A. Ali, Genetics similarity among four breeds of sheep in Egypt detected by RAPD
markers. African J. Biotech. 2 (7), pp. 194–197, ( 2003).
[23] I. Mastranestasis, C. H. Ligda, K. Theodorou, L. V. Ekateriniadou, Genetic structure and
diversity among three Greek sheep breeds using Random Amplified Polymorphic
DNA-PCR. J. of the Hellenic Vet. Med. Society. 62(4), pp. 301-313, ( 2011).
[24] M. Nei, Genetic distance between populations. American Naturalist. 106, pp. 283–292,
(1972.).
[25] M. Nei, Analysis of gene diversity in subdivided populations. P. Natl. Acad. Sci. USA.
70, pp. 3321-3323, (1973).
[26] R. C. Lewontin, The apportionment of human diversity. Evol. Biol. 6, pp.381-398,
(1972).
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