Determination of tetracycline hydrochloride in some pharmaceutical products using flow injection spectrophotometric techniquel

Shirwan Omer baban1 , Rasul Jameel Ali2

1 Chemistry Department, College of Education Science, Salahaddin University-Hawler, Iraqi-Kurdistan Region 2 Ministry of

Agriculture and Water Resources, Erbil

2 Clinical Biochemistry department, College of Health Sciences, Hawler Medical University, Hawler / Iraqi-Kurdistan Region

E- shirwan_baban@yahoo.co.uk

Received: 11 August. 2015;  Accepted: 30 August. 2015;  Published Online: 20 March. 2016

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ABSTRACT

A simple, rapid, and sensitive flow injection spectrophotometric system for determination of tetracycline hydrochloride (TCH) in pharmaceutical products. The method is based on diazotization of 2-aminopyridine and subsequent coupling with tetracycline hydrochloride in alkaline medium. The formed azo-dye showed maximum absorbance at 388 nm and the calibration curve in the linear range of 25.0-150.0 μg/ml of tetracycline hydrochloride with correlation coefficient 0.9988, while the detection limit was 10 μg/ml and sample frequency 123 samples/hour. The accuracy and precision data for determination of TCH by flow injection analysis method for triplicate injection at three concentration levels(25, 80 and 150 μg/ml) the relative standard deviation (RSD %) are 1.04, 0.247 and 1.75%, and relative error percent (Erel) are - 3.92, +1.06 and -1.9%. The proposed method was successfully applied to the determination of tetracycline hydrochloride in pharmaceutical products. The results obtained were in good agreement with those obtained by reference method.

KEYWORDS

Tetracycline hydrochloride; 2-aminopyridine; flow injection spectrophotometric

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