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Jalal H.S.Ismael, Hazha A.Mahmood
Dept. of Horticulture, Faculty of Agricultural Sciences, Univ. of Sulaimani
DOI: https://doi.org/10.17656/jzs.10313
Abstract:
Due to the fractionation of Hypericum triquetrifolium extract, it was found that the
isolated Hypericin gave the inhibition percentage of about 93.76% and the fungus
growth diameter average of 0.59 cm. The volatile oil extracted from Vitus agnus-castus
significant effect on the growth of the fungus about 71.20% inhibition percentage and
the fungus growth diameter average about 2.39 cm. However, the extraction of Nigella
sativa had a significant effect on the fungus growth about 82.03% and the fungus growth
diameter average about 1.43 cm due to 0.5 μl ml-1. For detection of secondary compound
products for H. triquetrifolium by HPLC technique, five compounds (peaks) were
detected, the higher percentage of the secondary compound, Hypericin, was 225.073 μg
ml-1, this is the active ingredient of this plant. An experiment was conducted on the
actual possibility of the highest concentration of the extracts, which gave the highest
percentage of inhibition of fungus Penicillium expansum by immersing the fruits of pear
(cv. Harme naska ) alone and mix with each other and found that the mixing process
has given impressive results, so mixtures of the last four gave the highest percentage
inhibition (100%) and on this basis can be relied upon recommendation, we can say that
the mixture (Hypericum + Nigella + Zingiber) gave a 100% . This treatment is better
because it does not exist in the composition of a chemical on the other hand give a
guarantee for those who work in organic cultivation.
Keywords: Disease Management, Blue Mold, Penicillium expansum , Plant extracts , HPLC
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