jzs-10794

Molecular characterization and phylogenetic analysis of Fasciola species in sheep and goats in Sulaymaniyah Province, Northern Iraq

Hana Sherzad Raoof 1*, Hardi Fattah Marif1, Heshu Sulaiman Rahman2, Mohammed Omar Baba Sheikh3, and Arkan Manucher San Ahmed3

1College of Veterinary Medicine, University of Sulaimani, Sulaimani, Kurdistan Region- Iraq

  2College of Medicine, University of Sulaimani, Sulaimani, Kurdistan Region- Iraq

3Directorate of Veterinary in Sulaymaniyah, Salim Street, Sulaimani, Kurdistan Region- Iraq

Original: 1 February 2020       Revised: 15 March 2020     Accepted: 5 April 2020        Published online: 20 June 2020  

Doi Link; https://doi.org/10.17656/jzs.10794

Abstract

Both F. hepatica and F. gigantica are considered as the main causes of human and animal fascioliasis that have much medical and economic importance worldwide. Nowadays, identification and description of Fasciola species using molecular-based techniques are critical and reliable approach in most laboratories and research centers. Thus, this research aimed to investigate the prevalence of fascioliasis and molecular characterization of isolated Fasciola species in sheep and goats in Sulaimaniyah province, Northern Iraq. Briefly, a total of 100 liver samples from slaughterhouse and 100 fecal samples from the animal field were collected from sheep and goats. In overall collected liver specimens, only 30 (15%) samples were found to be positive using polymerase chain reaction (PCR) assay, from which only 6 samples were selected for sequencing of the partial mitochondrial 28S rRNA gene and codon analysis. Simultaneously, the collected fecal samples were also analyzed using the Fecal Egg Count Reduction Test (FECRT), in which only 4 samples were positive. The results revealed that the identified four field sequences strains were F. hepatica and the other two field sequences were F. gigantica. In conclusion, we revealed that both F. hepatica and F. gigantica were distributed in Sulaimaniyah province and the mitochondrial 28S rRNA gene is confirmed as a potential biomarker in identifying various Fasciola species

Key Words: Liver fluke, tissue biomarker, small ruminants, abattoir, gene sequencing

 References

[1] Shahzad W, Mehmood K, Munir R, Aslam W, Ijaz M, Ahmad R, Khan MS, Sabir AJ."Prevalence and molecular diagnosis of Fasciola hepatica in sheep and goats in different districts of Punjab", Pakistan. Pakistan Veterinary Journal. Vol. 32, No. 4, pp. 535-538. (2012).

[2] Martinez-Valladares M, Rojo-Vazquez FA. "Intraspecific mitochondrial DNA variation of Fasciola hepatica eggs from sheep with different levels of anthelmintic resistance". Parasitology Research. Vol. 113, No. 7, pp. 2733-2741. (2014).

[3] Mas-Coma S, Bargues MD, and Valero MA. "Fascioliasis and other plant-borne trematodezoonoses". International Journal of Parasitology. Vol. 35, No. 11-12, pp. 1255–1278. (2005).

[4] Vaughan JL, Charles JA, and Boray JC. "Fasciola hepatica infection in farmed emus (Dromaiusn ovaehollandiae)". Australian Veterinary Journal. Vol. 75, No. 11, pp. 811–813. (1997).

[5] Charlier J, Vercruysse J, Morgan E, van Dijk J, Williams DJL. "Recent advances in the diagnosis, impact on production and prediction of Fasciola hepatica in cattle". Parasitology. Vol. 141, pp. 326–335. (2014).

[6] Mas-Coma S, Bargues M, Valero M. "Fascioliasis and other plant-borne trematodezoonoses". International Journal of Parasitology. Vol. 35, No. 11, pp. 1255-1278. (2005).

[7] Ashrafi K, Valero MA, Panova M, Periago MV, Massoud J, Mas-Coma S. "Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan". Iran Parasitology International. Vol. 55, pp. 249-260. (2006).

[8] Ai L, Chen MX, Alasaad S, Elsheikha HM, Li J, Li HL, et al. "Genetic characterization, species    differentiation and detection of Fasciola spp. by molecular approaches". Parasite Vectors. Vol. 4, No. 1, pp. 101-108. (2011).

[9] Teofanova D, Yoveva A, Radoslavov G, Hristov P. "Issues Associated with Genetic Diversity Studies of the Liver Fluke, Fasciola hepatica (Platyhelminthes, Digenea, Fasciolidae)". INTECH Open Access Publisher. pp. 251-274. (2012).

[10] Rokni M, MirhendiH, Behnia M, Jalalizand N. "Molecular characterization of Fasciola hepatica isolates by RAPD-PCR and ribosomal ITS1 sequencing". Iran Red Crescent Medical Journal.  Vol. 1, pp. 27-32. (2010).

[11] Mahdi NK, Al-Baldawi FA. "Hepatic fasciolosis in the abattoirs of Basrah Iraq". Annals of Tropical Medicine and Parasitology. Vol. l81, pp. 377–379. (1987).

[12] Sharma RL, Dhar DN, Raina OK. "Studies on the prevalence and laboratory transmission of fascioliasis in animals in the Kashmir valley". British Veterinary Journal. Vol. 145, No. 1, pp. 57-61. (1989).

[13] Over HJ, Jansen J, Van Olm PW. "Distribution and impact of helminth diseases of livestock in developing countries. Rome". FAO Animal Production and Health. (1992).

[14] Gargili A, Tüzer E, Gülanber A, Toparlak M, Efil I, Keles V, "Ulutas M. Prevalence of liver fluke infections in slaughtered animals in Trakya (Ihrace), Turkey". Turkish Journal of Veterinary and Animal Sciences. Vol. 23, pp. 115–116. (1999).

[15] Ashrafi K. "The Status of Human and Animal Fascioliasis in Iran: A Narrative Review Article". Iran Journal of Parasitology. Vol. 10, No. 3, pp. 306-328. (2015).

[16] Flanagan A, Edgar HW, Gordon A, Hanna RE, Brennan GP, Fairweather I. "Comparison of two assays, a faecal egg count reduction test (FECRT) and a coproantigen reduction test (CRT), for the diagnosis of resistance to triclabendazole in Fasciola hepatica in sheep". Veterinary Parasitology. Vol. 176, No. 2-3, pp. 170-176. (2011).

[17] Daniel R, Van Dijk J, Jenkins T, Akca A, Mearns R, Williams DJ. "Composite faecal egg count reduction test to detect resistance to triclabendazole in Fasciola hepatica". Veterinary Record. Vol. 171, No. 6. pp. 153-157. (2012).

[18] Baran AI, Saray HC, Katiraee F. "Molecular determination of Fasciola spp. Isolates from domestic ruminants fecal samples in the northwest of Iran". Iranian Journal of Parasitology. Vol. 12, No. 2, pp. 243-250. (2017).

[19] Alajmi RA. "Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep". Saudi Journal of Biological Sciences. Vol. 26, No. 1, pp. 112-117. (2019).

[20] Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W. "Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice". Nucleic Acids Research. Vol. 22, No. 22, pp. 4673-4680. (1994).

[21] Kumar S, Stecher G, Li M, Knyaz C, and Tamura K. MEGA X. "Molecular Evolutionary Genetics Analysis across computing platforms". Molecular Biology and Evolution. Vol. 35, pp. 1547-1549. (2018).

[22] Marcilla A, Bargues MD, Mas-Coma S. "A PCR-RFLP assay for the distinction between F. hepatica and F. gigantica". Molecular Cell Probes. Vol. 16, pp. 327–333. (2002).

[23] Ichikawa M, Itagaki T. "Discrimination of the ITS1 types of Fasciola spp. based on a PCR– RFLP method". Parasitology Research. Vol. 106, No. 3, pp. 757–761. (2010).

[24] Alajmi RA. "Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep". Saudi Journal of Biological Sciences. Vol. 26, No. 1, pp. 112-117. (2017).

[25] Mazyad SA and El-Nemr HI. "The endoparasites of sheep and goats, and shepherd in North Sinai Governorate, Egypt". Journal of Egyptian Society of Parasitology. Vol. 32, pp. 119-126. (2002).

[26] Tasawar Z, Munir U, Hayat CS and Lashari, MH. "Prevalence of Fasciola hepatica in goats around Multan". Pakistan Veterinary Journal.Vol. 27, No. 1, pp. 5-7. (2007).

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