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Nahla M. Saeed
Department of Microbiology, College of Veterinary Medicine, University of Sulemania.
DOI: https://doi.org/10.17656/jzs.10296
Abstract
A total of 311 Escherichia coli isolated from 460 healthy chickens at marketing age, and
56 E. coli isolated were obtained from 124 adult workers in broiler farms between April and
October 2013 -. Isolated E. coli were identified by cultural, biochemical, and Api E 20 test.
Antimicrobial susceptibility testing was determined by using the modified Kirby-Bauer’s disc
diffusion method. The antibiotic sensitivity result showed that the isolates were highly resistant
to Beta-lactam antibiotics. Among the 311 isolated E. coli from healthy chickens 264 (84.9%)
produced Beta-lactamase, while only 35 (62. 5%) of the 56 human isolates were found to
produce the enzyme. All of the 264 β-lactamase producer E. coli isolated from healthy chickens
and 35 β-lactamase producer E. coli isolated from human were tested for extended spectrum -
β-lactamase (ESBL) production, it was found that only 201 (76.1%) and 19 (54.3%), were
ESBL producers of E. coli isolated from healthy chickens and human respectively. Plasmids
were detected in all ESBL producer- E. coli (201) isolated from healthy chickens, which were
highly resistant to Beta-lactam antibiotics, while plasmid was detected only in 15 (78.9%) from
19 ESBLs producer E. coli isolated from human. By transformation the extracted plasmids into
E. coli DH5α; it was found that 165 (82.1%) Beta-lactamase genes from 201 isolates from
healthy chickens were carried by plasmid, while 10 (66.6%) Beta-lactamase genes from 15
plasmid isolated from human were carried by plasmid. The majority of these ESBL genes
(69.7%) were blaTEM and (10.9%) were blaCTX-M genes in healthy chickens, while in human the
majority of ESBL genes were (60%) blaTEM and (20. %) were blaCTX-M genes. Eleven isolated
from healthy chickens and one isolated from human did not contain blaTEM, blaSHV or blaCTX-M.
Keywords: - E. coli ; healthy chicken; human; Beta-lactamase; ESBL gene, plasmid
isolation.
REFERENCES
1. Johnson J. R., Kuskowski M. A., Smith K., O'BryanT., and Tatini T., "Antimicrobial-
resistant and extra-intestinal pathogenic Escherichia coli in retail foods" J. Infect. Dis.,
191, pp. 1040-1049 (2005).
2. Pfaller M. A., and Segreti J., "Overview of the epidemiological profile and laboratory
detection of extended-spectrum β-lactamases", Clin. Infect. Dis., 42, pp. 153–163,
(2006).
3. Livermore D. M ., "β-lactamases in laboratory and clinical resistance" J. Clin. Microbiol.
Rev., 8, pp. 557-584, (1995).
4. Bush K., Jacoby G. A., Medeiros A. A., "Functional classification scheme for β-
lactamases and its correlation with molecular structure" Antimicrob. Agen. Chemother.,
39, pp. 1211-1233, (1995).
5. Jouini A., Vinue L., Slama K. B., Saenz Y., Klibi N., Hammami S., Boudabous A., and
Torres C., "Characterization of CTX-M and SHV extended-spectrum β-lactamases and
associated resistance genes in Escherichia coli strains of food samples in Tunisia" J.
Antimicrob. Chemother. 60, pp. 1137-1141, (2007).
6. Blanc V., Mesa R., Saco M., Lavillad S., Pratsa G., Miró E., Navarroa F., Cortésa P.,
Llagosteraa M., " ESBL- and plasmidic class C β-lactamase-producing E. coli strains
isolated from poultry, pig and rabit farms" Vet. Microbiol., 118, pp. 299-304, (2006).
7. Jensen L. B. , Hasman H., Agersø Y., Emborg H. D. , Aarestrup F. M., "First description
of an oxyimin cephalosporin-resistant, ESBL-carrying Escherichia coli isolated from meat
sold in Denmark", J. Antimicrob. Chemother., 57, pp. 793-794, (2006).
8. Kojima A., Ishii Y., IshiharaK, Esaki H., Asai T., Oda C., Tamura Y., Takahashi T., and
Yamaquchi K., " Extended-spectrum β-lactamase-producing Escherichia coli strains
isolated from farm animals from 1999 to 2002" J. Antimicrob. Agen. Chemother. 49, pp.
3533-3537, (2005).
9. Yan J. J., Hong C.Y., Ko W.C., Chen Y. J., Tsai S. H., Chuang C. L., and Wu J. J.,
"Dissemination of BlaCMY-2 among Escherichia coli isolates from food animals, retail
ground meats, and humans in Southern Taiwan" Antimicrob. Agents. Chemother., 48, pp.,
1353-1356 (2004).
10. Saeed N. M., "Investigation into prevalence of Escherichia coli infection and some other
diseases in broiler farms in Sulaimani district" M.V.Sc., thesis, College of Veterinary
Medicine, Sulaimania University, Iraq, (2004).
11. Saeed N. M., Aref E. T., and Al-Obaidi R. M., " Study of Beta-lactamase and Extended-
Spectrum Beta-lactamase Production by Escherichia coli in Broiler Farms in Sulaimania
Province", J. Tik. Univ. for Agri. Sci., 13 (3), pp. 60-66, (2013).
12. Calnek B., Barnes W., Charles H. J., Beard W., McDougald L. R., and Saif, Y. M.,
"Diseases of Poultry", 10th
Ed., lowa State University Press, USA, Chap. 18, (1997).
13. Cowan S.T., "Cowan and Steel’s manual for identification of medical bacteria" 2nd
Ed.,
Cambridge University Press, Cambridge, UK, pp. 138-139. (1985).
14. Colle J. G., Fraser A. G., Marmiom B. P., and Simmons A., " Practical medical
microbiology", 14th Ed., Churchil Livingstone, USA, (1996).
15. CLSI "Performance Standards for Antimicrobial Susceptibility Testing", 17th
Informational Supplement, M100-S17. Wayne, PA: Clinical and Laboratory Standards
Institute, (2007).
16. Kizirgil A., Demirdag K., Ozden M., Bulut Y., Yakupogullari Y., and Toraman Z. A., "
In vitro activity of three different antimicrobial agents against ESBL producing
Escherichia coli and Klebsiella pneumoniae blood isolates" J. Microbi. Res.160(2), pp.
135-140, (2005).
17. Sambrook J. and Russell D. W., "Molecular Cloning: A Laboratory Manual, Cold Spring
Harbor Laboratory", Cold Spring Harbor, NY. (2001).
18. Miranda J. M., Guarddon M., Vazquez B. I., Fente C. A., Barros-Velazquez J., Cepeda
A., and Franco C. M., "Evolution of resistance of poultry intestinal Escherichia coli
during three commonly used antimicrobial therapeutic treatments in poultry" Poul. Sci.,
87(8), pp. 164-168, (2008).
19. Hu G. Z., Zhang C. H., Yuan L., Yang Y. R., and Liang J., " Detection of β-lactamase
and extended-spectrum β-lactamase of pathogens isolated from pig and chicken and their
antibiotic susceptibility test" J. Agric. Sci. China., 4, pp. 877–882, (2005).
20. Ramphal R., and Ambrose P. G., "Extended-spectrum β-lactamases and clinical
outcomes: current data", Clin. Infect. Dis., 42, pp. 164– 172, (2006).
21. Liu J. H., Wei S. Y., Ma J. Y., Zeng Z. L., Lü D. H., Yang G. X. and Chen Z. L.,
"Detection and characterisation of CTX-M and CMY-2 β-lactamases among Escherichia
coli isolates from farm animals in Guangdong Province of China", Int. J. Antimicrob.
Agen., 29, pp.576–581, (2007).
22. Smet A., Martel A., Persoons D., Dewulf J., Heyndrickx M., Catry B., Herman L.,
Haesebrouck F., and Butaye P., "Diversity of extended-spectrum β-lactamases and class C
β-lactamases among cloacal Escherichia coli isolates in Belgian broiler farms" J.
Antimicrob. Agen. Chemother. 52, pp. 1238-1243, (2008).
23. Mesa R.J., Blanc V., Blanch A.R. , Cortés P., González J.J., Lavilla S., Miró E., Muniesa
M., Saco M., Tórtola M.T., Mirelis B., Coll P., Llagostera M., Prats G., and Navarro F.,
"Extended-spectrum β-lactamase-producing Enterobacteriaceae in different environments
(humans, food, animal farms and sewage)", J. Antimicrob. Chemother., 58(1), pp. 211-
215, (2006).
24. Overdevest I., Willemsen I., Rijnsburger M., Eustace A., Xu L., Hawkey P., Heck M.,
Savelkoul P., Vandenbroucke-Grauls C., van der Zwaluw K., Huijsdens x., and
Kluytmans j., "Extended-spectrum β-lactamase genes of Escherichia coli in chicken meat
and humans, The Netherlands" Emerg. Infect. Dis., 17, PP. 1216-1222, (2011).
25. Agerso Y., Aarestrup F.M., Pedersen K., Seyfarth A. M., Struve T., and Hasman H.,"
Prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in
Danish slaughter pigs and retail meat identified by selective enrichment and association
with cephalosporin usage" J. Antimicrob. Chemother., 67, pp.582-588, (2012).
26. Levy S.B., FitzGerald G.B., Macone A.B., "Spread of antibiotic-resistant plasmids from
chicken to chicken and from chicken to man", Nature., 260, pp.40-42, (1975).
27. Johnson J. R., Sannes M. R., Croy C., Johnston B., Clabots C., Kuskowski M. A.,
Bender J., Smith K. E., Winokur P. L., and Belongia E. A., "Antimicrobial drug-
resistant Escherichia coli from humans and poultry products, Minnesota and Wisconsin,
2002–2004" Emerg. Infect. Dis., 13, pp. 838-846, (2007).
28. Vincent C., Boerlin P., Daignault D., Dozois C. M., Dutil L., Galanakis C., Reid-Smith
R. J., Tellier P., Tellis P. A., Ziebell k., and Manges A. R., "Food reservoir
for Escherichia coli causing urinary tract infections" J. Emerg. Infec. Dise. 16, pp. 88-95,
(2010).
29. Leverstein-van Hall M. A. , Dierikx C. M. , Cohen S. J., Voets G. M., van den Munckhof
M. P., van Essen-Zandbergen A., Platteel T., Fluit A.C., van de Sande-Bruinsma
N., Scharinga .J, Bonten M.J., Mevius D.J., "Dutch patients, retail chicken meat and
poultry share the same ESBL genes, plasmids and strains" Clin. Microbiol. Infect., 17(6),
pp. 873-880, (2011).
30. Ryoo N. H., Kim E. C., Hong S. G., Park Y. J., Lee K., Bae K., Song E. H., and Jeong
S. H., "Dissemination of SHV-12 and CTX-M-type extended-spectrum β-lactamases
among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of
GES-3 in Korea", J. Antimicrob. Chemother., 56, pp.698–702, (2005).
31. Carattoli A., García-Fernández A., Varesi P., Fortini D., Gerardi S., Penni A., Mancini C.
and Giordano A., "Molecular epidemiology of Escherichia coli producing extended-
spectrum β-lactamases isolated in Rome, Italy" J. Clin. Microbiol. 46, pp. 103–108,
(2008).
32. Kiratisin P., Apisarnthanarak A., Laesripa C., and Saifon P., "Molecular characterization
and epidemiology of extended-spectrum-β-lactamase-producing Escherichia
coli and Klebsiella pneumoniae isolates causing health care-associated infection in
Thailand, where the CTX-M family is endemic", Antimicrob. Agen.
Chemother., 52, pp.2818–2824, (2008).
33. Smet A., 1., Rasschaert G., Martel A., Persoons D., Dewulf J., Butaye P., Catry
B., Haesebrouck F., Herman L., and Heyndrickx M., " In situ ESBL conjugation from
avian to human Escherichia coli during cefotaxime administration" J. Appl. Microbiol.
110, pp. 541-549, (2011).
34. Paterson D. L., and Bonomo R. A., " Extended-spectrum β-lactamases: a clinical update"
J. Clin. Microbiol. Rev., 18, pp. 657–686, (2005).
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