Tips for Skill 32

This skill will require you to culture and identify common dermatophytes. Upon completion of this skill, you will be able to prepare and interpret a DTM plate and use an ultraviolet light for identification of ringworm while following OSHA guidelines for proper PPE.

Tips for materials that need to be submitted:

• Logbook with description of procedures performed

  • You need a description of the procedures performed. Describe in detail in your own words how you performed the tests

    • Describe in detail how you collected the sample and inoculated the DTM

    • Describe the use of a Wood's lamp

    • Do not forget to discuss how you collected the sample from the plate for microscopic identification

  • You need to perform the tests on two patients.

  • You need to include patient info in your log of results (signalment)

  • You need to include the results for each patient

    • Don't forget to include what you saw under the microscope

• Photos of DTM showing colony growth and/or color change for two patients

  • The photos should be the DTMs for the two patients you discussed in your log book and the observation records for both

  • The DTMs need to be labeled with:

    • The patient name

    • Date of inoculation

    • If the label will block the growth you are trying to show write the info on a piece of paper and have it in the picture next to the DTM

• Daily observation record and final test results for two patients

  • Make sure you have a full patient signalment

  • Record your daily observations as to what you see including:

    • Growth (character, size, depth)

    • Color change

• Helpful resources in addition to your textbooks and study materials:

  • http://veterinarymedicine.dvm360.com/how-perform-and-interpret-dermatophyte-cultures