F620

Tanawin Kawsuwan, Natchuda Jaipieam

Advisor Dr. Singtoe Boonrotpong, Rochanakorn Kongseng

Abstract

Thailand located in a tropical climate. In this climate, many mosquitoes can lay eggs and breed well, which makes it possible to find a large number of mosquitoes. One of the mosquitoes found was the Anopheles species. Anopheles mosquitoes are one of the most widespread malaria vectors in Thailand. In some groups cannot be clearly indicated that they are main malaria vectors. This project has been studied to determine the genetic diversity of Anopheles nigerrimus and An. nitidus in Hatyai district in Songkhla Province using cytochrome c oxidase I (COI), which is one of the most popular markers used for molecular systematics. Fragments of this gene are often used to infer phylogenies, particularly the region near the 5'-end, which is used by the DNA Barcoding Consortium. In this study, genetic diversity and phylogeny was showed that both species were monophyletic groups and same origin between population. However, genetic variations were significantly difference between species.