E619

Baibua Borkird, Montraluk Prommanee

Advisor Assoc. Prof. Dr. Jirat Meesane, Adinan Jehsu, Jutakan Thonglam, Yadanar Mya Moe

Abstract

In present day, synthetic biomedical material are used for tissue engineering, especially in the wound healing. The commercial membrane becomes an alternative way and it has a high ability for new tissue formation. However, this membrane has high cost for patient implantation.

In this study choose alginate (complex carbohydrate is extracted from brown sea weed) that is common and cheap. The research of Burapha University and Silpakorn University, Applications of Polymers from Marine Resource, shows that alginate is able to inhibit inflammation and bacteriostatic action. Polyvinyl alcohol usually is used for welding substance to be dense. Alginate and Polyvinyl alcohol (PVA) are polymers that can fabricate into membrane form. Moreover, the biofunctionality of these polymers support for cell migration and proliferation. In this study, the 5% by mass per unit volume of alginate and PVA is combined at different ratio including 100:0, 70:30, 50:50, 30:70 and 0:100 3 ml per piece in square mold that is examined 15 pieces each ratio. The freeze thawing method at -80 degree celsius is selected for membrane fabrication and Freeze dry is conserved qualification of membrane. Calcium Chloride was used for soaking the membrane in the last process to improve the mechanical property of the membrane. This productive process is tested for following cell growth. Photomicroghaphy can be recorded from using confocal microscope. Finally, overall are utilized to prepare the most effective for cell growth that is a principle part of wound healing.

The 70:30 of Alginate/PVA showed the good performance for wound healing application. The concentration of solution results in forming. The first experiment, which is mixed alginate solution with a concentration of 1%w/v and the PVA solution is concentrated. The mass to volume ratio, which defines the process leading to a freeze thow result is a mixture of such a change is a gel but has not been used enough in a gel adhesive.