Protein Fold Change
Protein Fold Change filters a protein table according to the peptides fold change (FC) of the proteins. If at least x % of the peptides are above or below a target FC, then the protein is selected. By default Protein Fold Change selects all the proteins with at least 80% of their peptides with a FC > 2 or FC <0.5.
Manual :
1- install Julia free programming language and install package :
DataFrames : Pkg.add("DataFrames")
2- unzip the software
3- edit config file in the config directory and enter the names of :
# column name of proteins
protRow=PG_ProteinNames
# column name of peptides
pepRow=EG_PrecursorId
# column name of fold change
FCRow=FC
4- copy your n tables in csv (TAB delimitated by default) files in the “data” directory.
- first line of the table : column names with at least 3 columns : protein names, peptide names and FC
- next lines : values
5- execute the software by the command : julia software_name
6- results tables are in the results directory.