Log2 transformation

For each numerical values Xi of a table, this software perform the transformation into log base 2 : ln(Xi)/ln(2) or ln(Xi+1)/ln(2). For a more general usage, see also Process Tables.

Manual :

1- if not already installed, install Perl free programming language.

2- unzip the software

3- copy your microarray files in the “data” directory. The files must be formated as follows (an example is given with the soft) :

- txt or csv files with TAB separator

- first line : column names

- first column : probset names or gene names

- second column : definitions / comments

- next columns : gene expression values with dot as decimal separator. The first columns must contain expression values for the controls. The last columns must contain expression values for the second group.

4- execute the software by the command : perl software_name or double click on the .pl file. The log2_plus_1 script add +1 before log2 transformation

5- processed files are in the “results” directory.