PepAlign

PepAlign takes list(s) of peptides as input and aligns the peptides on one (or more) Fasta sequences. PepAlign can calculate the protein sequence coverage, the post-translational modification (PTM) coverage and the list of PTMs positions for any PTM.

peptides list

⬇

=======================

PepAlign Results

=======================

Sequence Coverage : 80.0 %

PTMs Coverage (STY) : 66.67 %

Protein sequence :

MLKFKYGARN PLDAGAAEPI ASRASRLNLF FQGKPPFMTQ QQMSPLSREG

--|---- ---------- -|--|----- --------| + + --

(...)

Manual :

1- install Julia v1.0.3 or JuliaPro 1.0.3 free programming language

2- unzip the software

3- copy your fasta sequence(s) in the fasta directory

4- copy your peptides list(s) in text files in the peptides directory, one peptide / row

for example :

5- execute the software by the command : julia software_name

6- results tables are in the table directory.

7- sequence alignement are in the results directory,

peptides and PTMs tables are in the table directory,

PTMs positions are listed in the PTMsites directory.