The Potential of Andrographis Paniculata (Serpentina) with Wild Honey as a Tea Against Escherichia Coli (E. Coli) 

Statement of the Problem

1. What is the potential of Andrographis Paniculata as a raw material for making tea, with the addition of honey?

2. How do the antimicrobial properties of Andrographis Paniculata, with the addition of honey, compare against Escherichia coli?

3. What are the potential risks and benefits of using Andrographis Paniculata, with the addition of honey, as a natural antimicrobial agent compared to modern medicines?

4. What is the appropriate dosage of Andrographis Paniculata, with the addition of honey, to maximize their antimicrobial effects while ensuring safety?

Methods

This study involves experimenting to investigate the inhibitory effects of Paniculata extract on the growth of E. coli bacteria. The experiment will follow a standardized protocol. Firstly, a pure culture of E. coli bacteria will be obtained. Paniculata extract will be prepared using a standardized hot water extraction method and diluted according to mass of crushed leaves. The E. coli culture will be divided into four petri dishes, each labeled with the corresponding Paniculata extract concentration. Inoculation will be performed by adding a standardized amount of E. coli bacteria to each sample. Four samples will be tested according to the amount of concentration and with the addition of wildflower honey will be tested against a standardized amount of E. coli for an accurate monitoring. The samples will be incubated at an appropriate temperature for E. coli growth for a predetermined period. After incubation, the presence of bacterial growth in each sample will be observed and recorded. If applicable, the diameter of bacterial colonies will be measured to quantify the inhibition of growth. The collected data, including Paniculata extract concentration, presence/absence of bacterial growth, and colony diameter, will be analyzed statistically to determine the percentage of inhibition of E. coli growth for each Paniculata extract concentration. Ethical considerations will be followed to ensure compliance with guidelines for research involving living organisms and necessary approvals will be obtained.

Results and Discussion

In this study, the evaluation of Andrographis Paniculata extract against Escherichia coli revealed a lack of antimicrobial activity, despite stringent inoculation procedures and methodological rigor. Notably, the positive control, chloramphenicol at 30 micrograms, exhibited antimicrobial efficacy. This unexpected outcome suggests that Andrographis Paniculata may not possess the anticipated antimicrobial properties against the specific strain of E. coli tested in the concentrations utilized. Further investigations are warranted to explore potential dose-dependent responses, variability in antimicrobial properties, and strain-specific susceptibilities to elucidate the underlying factors contributing to the observed results. These findings underscore the complexity of herbal antimicrobial efficacy and emphasize the need for continued research to comprehensively understand the antimicrobial potential of Andrographis Paniculata against E. coli. 

In this study, the evaluation of Andrographis Paniculata extract against Escherichia coli revealed a lack of antimicrobial activity, despite stringent inoculation procedures and methodological rigor. Notably, the positive control, chloramphenicol at 30 micrograms, exhibited antimicrobial efficacy. This unexpected outcome suggests that Andrographis Paniculata may not possess the anticipated antimicrobial properties against the specific strain of E. coli tested in the concentrations utilized. Further investigations are warranted to explore potential dose-dependent responses, variability in antimicrobial properties, and strain-specific susceptibilities to elucidate the underlying factors contributing to the observed results. These findings underscore the complexity of herbal antimicrobial efficacy and emphasize the need for continued research to comprehensively understand the antimicrobial potential of Andrographis Paniculata against E. coli.

Results and Discussion

In this study, the evaluation of Andrographis Paniculata extract against Escherichia coli revealed a lack of antimicrobial activity, despite stringent inoculation procedures and methodological rigor. Notably, the positive control, chloramphenicol at 30 micrograms, exhibited antimicrobial efficacy. This unexpected outcome suggests that Andrographis Paniculata may not possess the anticipated antimicrobial properties against the specific strain of E. coli tested in the concentrations utilized. Further investigations are warranted to explore potential dose-dependent responses, variability in antimicrobial properties, and strain-specific susceptibilities to elucidate the underlying factors contributing to the observed results. These findings underscore the complexity of herbal antimicrobial efficacy and emphasize the need for continued research to comprehensively understand the antimicrobial potential of Andrographis Paniculata against E. coli. 

In this study, the evaluation of Andrographis Paniculata extract against Escherichia coli revealed a lack of antimicrobial activity, despite stringent inoculation procedures and methodological rigor. Notably, the positive control, chloramphenicol at 30 micrograms, exhibited antimicrobial efficacy. This unexpected outcome suggests that Andrographis Paniculata may not possess the anticipated antimicrobial properties against the specific strain of E. coli tested in the concentrations utilized. Further investigations are warranted to explore potential dose-dependent responses, variability in antimicrobial properties, and strain-specific susceptibilities to elucidate the underlying factors contributing to the observed results. These findings underscore the complexity of herbal antimicrobial efficacy and emphasize the need for continued research to comprehensively understand the antimicrobial potential of Andrographis Paniculata against E. coli.