Tu-Ky LY
Contact
INERIS, MIV/TEAM, MIV/ESMI
UMR-I 02 SEBIO
Parc ALATA, BP2
5, rue Taffanel
60550 Verneuil en Halatte, France
+33 (0) 3 44 55 66 49
PhD Project
Modelling of the Adverse Outcome Pathway (AOP) leading to fecundity inhibition in zebrafish: from initiating events to population impacts
The translation of subtle functional deficits within individuals into population-level effects is identified as a main challenge for the hazard and risk assessment of endocrine disruptor (ED) compounds in fish. Data collected at the different biological levels of organization could be integrated into a pathway-based toxicological framework (Adverse Outcome Pathway, AOP) using a modelling approach. This implies establishing and modelling functional and quantitative links between a molecular initiating event and population-level effects, with the final goal of supporting regulatory decision-making regarding EDs.
One of the mechanisms of action of endocrine disruption often studied is the disruption of the gonadal cytochrome P450 aromatase because of its involvement in the reproductive process. The experimental data and mechanistic models already available at INERIS for the zebrafish could support the development and/or improvement of quantitative AOP for EDs leading to a decrease in the fecundity, mediated by an inhibition of aromatase A or by other molecular initiating events.
The aim of my PhD will therefore be the development and improvement of multi-scale models of toxicity pathways enabling to more accurate predictions of ED impacts on the populations.
Poster_sefa_TKL_V3.pdfWork experiences
October 2021
(in progress)
Ph.D Student in Ecotoxicology and Toxicokinetic Modelling
Supervisors: Dr. Rémy Beaudouin, Dr. Nathalie Hinfray
INERIS, Modelisation and Experimental Toxicology (TEAM) - Ecotoxicology of Substances and Environments (ESMI) - UMR-I 02 SEBIO
Project: Modelling of the AOP leading to fecundity inhibition in zebrafish.
Verneuil-en-Halatte, France
September 2018 October 2021
Student Pharmacist
Several Community Pharmacies in Paris, France
Diagnosis of common diseases
Presciptions dispensation
Counselling a drug delivery
Pharmacovigilance
Paris, France
January 2021
July 2021
Research Intern in Neurotoxicology
Supervisors: Dr. Chrystelle Ibanez, Dr. Léo Macé
IRSN, Laboratory of radiotoxicology and radiobiology (LRTOX)
Project: Metal tungsten toxicity: effects of tungsten particle inhalation on rat brain, the implication of oxidative stress and neuroinflammation.
"Nose Only" exposure of rats, micro-dissection of rat brain
WB, immunohistochemistry, microscopy and microphotography analysis
Statistical analysis (R studio)
Fontenay-aux-Roses, France
April 2020
September 2020
Research Intern in Toxicology
Supervisor: Dr. Pascal Loyer
Institut NuMeCan, INSERM UMR1241
Project: In vitro study of the effects of a low dose mixture of pesticides on liver cells in vitro: cytotoxic and steatogenic effects.
State of the art of knowledge between pesticide exposure and NAFLD
Analysis of the results obtained by the team on the effects of pesticides in the HepaRG hepatoma cells
Proposal of experimental design tor the in vitro study of the effects of pesticides on the hepatocyte steatosis
Rennes, France
September 2019
January 2020
Pharmacy Residency
Supervisors: Dr. Vanessa Bloch, Dr. Clément Delage, Dr. Alexia Frapsauce
AP-HP, Hôpital Lariboisière-Fernand-Widal
Geriatric unit, Medical unit specializing in addiction, and Psychiatric unit:
Validation of prescriptions
Pharmacovigilance
Risk reduction during hospitalization
Paris, France
July 2018
November 2018
Research Intern in Clinical Research
Supervisors: Pr. Anh-Tuan Dinh Xuan, Dr. Huy Thong Hua
AP-HP, Hôpital Cochin Port-Royal
Cystic Fibrosis and Chronic Lung Disease: Biopathology and Clinical phenotypes (UPRES EA 2511)
Project: Advantage of the measure of nasal NO in the diagnose of Primary ciliary dyskinesia.
Population sampling according to defined criteria
Measurement of nasal NO
Respiratory function tests
Statistical analysis (GraphPad)
Paris, France
Education and training
2020-2021
Master’s Degree (2nd Year) in Human Toxicology, Risk Assessment, Vigilance (THERV)
Graduated with honors
Université de Paris & Université Paris-Saclay (Paris, France)
2016-2021
Pharm.D Candidate, Thesis in Progress
Leading to pharmaceutical industry and research, specializing in toxicology
Faculté de Pharmacie de Paris (Paris, France)
2018 - 2019
Master’s Degree (1st Year) in Pharmacology, Physiology, and Toxicology (PPT)
Graduated with honors
Université de Paris (Paris, France)
2014 - 2016
Common 1st Year of Health Studies (PACES)
Competitive exam leading to medicine, pharmacy, dentistry and maieutic
Global Ranking: 100/890
Université de Paris (Paris, France)
Language skills and Software
French: mother tongue
Vietnamese: mother tongue
English: fluent (C1)
German: basics (B1)
Software: Pack Office, R-studio, GraphPad
Publications
Delage C, Darnaud L, Etain B, Vignes M, Ly T-K, Frapsauce A, Veyrier M, Delavest M, Marlinge E, Hennion V, Meyrel M, Jacob A, Chouchana M, Smati J, Pataud G, Khoudour N, Fontan J-E, Labat L, Bellivier F, Lloret-Linares C, Declèves X, Bloch V. (2022). Cytochromes P450 and P-Glycoprotein Phenotypic Assessment to Optimize Psychotropic Pharmacotherapy: A Retrospective Analysis of Four Years of Practice in Psychiatry. Journal of Personalized Medicine. 12(11):1869. https://doi.org/10.3390/jpm12111869