! Lectures of R Milo

6 Лекций Мило по Биологии клетки в числах за 2013 и 2014 гг.:

https://www.youtube.com/channel/UChCzuzoZp5NheAPWH5YoGWw

на английском языке.

Содержание лекций.

Пользователь Cell Biology By The Numbers добавил видео Год назад

Опубликовано: 7 янв. 2015 г. Class 2014-2015

Lecture 6: Information and Errors - Genomes & Mutations

* What is the correlation between genome size and gene number in different organisms?

* How many genes does a Vibrio cholerae have?

* How many mutations occur in a replication of the E. coli genome?

* How many proteins have a translational error in them?

* What is the best strategy to get a bacteria with a specific point mutation?

* If we are looking for a newly arising mutation in the culture of humans on earth, will we have it?

* How likely is it to find a person with two specific point mutations de novo?

Пользователь Cell Biology By The Numbers добавил видео Год назад

Опубликовано: 31 дек. 2014 г. Class 2014-2015

Lecture 5: Rates and durations

* What is faster in bacteria, transcription or translation?

* How long does it take to produce a functional protein?

* How long does it take to diffuse across the cell?

* How long does it take to diffuse across axon of 1 cm length?

Пользователь Cell Biology By The Numbers добавил видео Год назад

Опубликовано: 28 дек. 2014 г. Class 2014-2015

Lecture 4: Energies and Forces

* What is the characteristic power consumption of a human?

* How much land is needed to supply the food that will give us the needed power?

* How much power consumed per cell?

* How many ATP-equivalents consumed per cell?

* What is a cell ATP spent on?

* If the average human cell spent ALL of its energy on protein synthesis (deg for free), what would be the average turnover?

Пользователь Cell Biology By The Numbers добавил видео Год назад

Опубликовано: 17 дек. 2014 г. Class 2014-2015

Lecture 3: Concentrations and absolute numbers

* How many free H+, H3O+ (hydronium/protons ions) are in a bacterial cell volume?

* If I have one molecule in a bacterial cell volume, what is the concentration?

* How many water molecules per bacterial cell? Per mammalian cell?

* How does a water molecule feels in a cell? In a analogy to a bee...

* If you circle the globe with white golf balls, and put a black ball one in a billion, how many will be in the world?

* Your chance to win the Lottery can be 1:100,000,000. Let’s say you stack them all up and have to blindly choose the right one. How tall is the stack?

Пользователь Cell Biology By The Numbers добавил видео Год назад

Опубликовано: 9 дек. 2014 г. Class 2014-2015

Lecture 2: Size, mass and geometry

* What is heavier, mRNA (spliced) or the protein it codes for?

* How many atoms in the human body?

* In overnight culture of bacteria (E. coli, 0.2% sugar, saturated), what fraction of media volume are cells?

Пользователь Cell Biology By The Numbers добавил видео Год назад

Опубликовано: 4 дек. 2014 г. Class 2014-2015

Lecture 1: Quantitative reasoning in molecular and cell biology

* Course introduction

* How much time will take you (as an experienced sprinter) to respond in a 100m race to the referee pistol?

* How many proteins are in an E. coli cell?

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Over the past decades, biology has evolved rapidly from a descriptive, qualitative discipline to a more analytical, data-driven and quantitative one. Our ability to collect numbers that describe the most basic molecular processes within the cell has increased significantly, and simple calculations based on these data can provide important insights and enrich our scientific intuition.

This course is aimed at exposing students to the practice of making back of the envelope calculations (so called Fermi problems) with key numbers in biology, and its useful applications in research. We will learn how to identify the major factors that determine the order of magnitude of the results, when to allow simplification, how to calculate them efficiently, and how to avoid common pitfalls.

Prof. Ron Milo, Department of Plant Sciences, Weizmann Institute of Science