For your information.
Niwa and his colleagues examined the relation between antibiotics concentration and expression level. This is very nice paper!
Kinetics of drug selection systems in mouse embryonic stem cells.
Yuhki Nakatake, Setsuko Fujii, Shinji Masui, Toshimi Sugimoto, Satomi Torikai-Nishikawa, Kenjiro Adachi and Hitoshi NiwaEmail author
BMC Biotechnology 201313:64
Puromycin
Stock concentration 1 mg/mL in DDW
Working concentration 0.5-3 ug/mL (usually 1-2 ug/mL)
1-2 days to start killing mammalian cells.
1日くらいで細胞が死に始める。
最も安価で濃度依存的に発現量が上がる。
Link: InVivoGen
Blasticidin S
Stock concentration 10 mg/mL in DDW
Working concentration 1-30 ug/mL (usually 10 ug/mL)
2-3 days to start killing mammalian cells.
Link: InVivoGen
Zeocin
Stock concentration 100 mg/mL in DDW
Working concentration 0.5-1000 ug/mL (usually 100 ug/mL)
4-5 days to start killing mammalian cells.
Expensive.
Link: InVivoGen
Hygromycin
Stock concentration 50-100 mg/mL in DDW
Working concentration 100-1000 ug/mL (usually 100-200 ug/mL)
4-5 days to start killing mammalian cells.
Link: Hygromycinのストック作製
G418
Stock concentration 50-100 mg/mL in DDW (acidic)
Working concentration 100-1000 ug/mL (usually 700-1000 ug/mL)
6-7 days to start killing mammalian cells.
Purchased from Nacalai Tesque. 23985-94