- Digest the plasmid vector by restriction enzyme of interest sufficiently.
- Subject to gel electrophoresis and gel purification of the vector fragment.
- We recommend to purify the fragment for efficient BAP reaction.
- Add BAP buffer and BAP, and incubate it at 50 degree C for 30 min.
- For example.
- 10 uL: Purified DNA fragment
- Add 7 uL DDA
- Add 2 uL 10x BAP buffer
- Add 1 uL BAP
- Subject to gel electrophoresis and gel purification of the vector fragment.