BAP treatment

  1. Digest the plasmid vector by restriction enzyme of interest sufficiently.
  2. Subject to gel electrophoresis and gel purification of the vector fragment.
    • We recommend to purify the fragment for efficient BAP reaction.
  3. Add BAP buffer and BAP, and incubate it at 50 degree C for 30 min.
    • For example.
      • 10 uL: Purified DNA fragment
      • Add 7 uL DDA
      • Add 2 uL 10x BAP buffer
      • Add 1 uL BAP
  4. Subject to gel electrophoresis and gel purification of the vector fragment.