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Protocols in Aoki lab, NIBB
Home
1. Reagents, drugs, antibiotics, etc
10xLB
Antibiotics for mammalian cells
DNA電気泳動用ゲル
LB plate, LB medium
MMI
SOB、SOC
2. Molecular biology
BAP treatment
Oligo DNA annealig and ligation
3. Biochemistry
4. Cell biology
AAV
AAV Transfection Protocol
AAV精製
Coating glass surface
Giemsa stain
HaloTag染色
Lipofection
Passage of cultured cells
poly-Hemaコーティング
5. Microscopy, imaging
TOKAIHIT gas regulator
6. Software, programming, image analysis
Benchling
gRNA design
CellDesigner
Tips for CellDesigner
ImageJ/Fiji
Edit Lut
Tips for ImageJ/Fiji
Tracking by TrackMate in ImageJ/Fiji
MATLAB
FCS/FCCS analysis by MATLAB
MATLABのFCS/FCCS解析プログラム
FCCSanalysis_PCNC140805スクリプト
FCCSanalysis_PCNCKdスクリプト
Func_AcXcFit関数
Func_correlation関数
Func_detrend関数
Func_dirNumber関数
Func_Fitting関数
Func_tifToTimecourse関数
Image processing by MATLAB
FRETImageProcess2クラス
Adjust
AlignStack
CheckIntensityAroundNucleus
FigureBackground
FRET画像解析例
GetImage
GetTimeOfImage
IntensityHistogram
ModifySortArray
Movie
MovieFromTiff
OutputCAAXIntensity
OutputNESIntensity
OutputNLSIntensity
RatioImage
RatioImageFromTiff
ReadNDFile
ReadTiffFile
RegionForOutput
RegionGrowing
Segmentation
SortNLSNESCAAX
ソースコード
Tiffファイルの扱い方
TrackMate2MATLAB
基本事項
画像の表示と出力
顕微鏡画像読み込み
Tips for MATLAB
7. Python
Environment setup
Find RE site from AA sequence by Python program
Memo
Virtual environment
Visualization
Grid of Subplots
8. Others
Protocols in Aoki lab, NIBB
Home
1. Reagents, drugs, antibiotics, etc
10xLB
Antibiotics for mammalian cells
DNA電気泳動用ゲル
LB plate, LB medium
MMI
SOB、SOC
2. Molecular biology
BAP treatment
Oligo DNA annealig and ligation
3. Biochemistry
4. Cell biology
AAV
AAV Transfection Protocol
AAV精製
Coating glass surface
Giemsa stain
HaloTag染色
Lipofection
Passage of cultured cells
poly-Hemaコーティング
5. Microscopy, imaging
TOKAIHIT gas regulator
6. Software, programming, image analysis
Benchling
gRNA design
CellDesigner
Tips for CellDesigner
ImageJ/Fiji
Edit Lut
Tips for ImageJ/Fiji
Tracking by TrackMate in ImageJ/Fiji
MATLAB
FCS/FCCS analysis by MATLAB
MATLABのFCS/FCCS解析プログラム
FCCSanalysis_PCNC140805スクリプト
FCCSanalysis_PCNCKdスクリプト
Func_AcXcFit関数
Func_correlation関数
Func_detrend関数
Func_dirNumber関数
Func_Fitting関数
Func_tifToTimecourse関数
Image processing by MATLAB
FRETImageProcess2クラス
Adjust
AlignStack
CheckIntensityAroundNucleus
FigureBackground
FRET画像解析例
GetImage
GetTimeOfImage
IntensityHistogram
ModifySortArray
Movie
MovieFromTiff
OutputCAAXIntensity
OutputNESIntensity
OutputNLSIntensity
RatioImage
RatioImageFromTiff
ReadNDFile
ReadTiffFile
RegionForOutput
RegionGrowing
Segmentation
SortNLSNESCAAX
ソースコード
Tiffファイルの扱い方
TrackMate2MATLAB
基本事項
画像の表示と出力
顕微鏡画像読み込み
Tips for MATLAB
7. Python
Environment setup
Find RE site from AA sequence by Python program
Memo
Virtual environment
Visualization
Grid of Subplots
8. Others
More
Home
1. Reagents, drugs, antibiotics, etc
10xLB
Antibiotics for mammalian cells
DNA電気泳動用ゲル
LB plate, LB medium
MMI
SOB、SOC
2. Molecular biology
BAP treatment
Oligo DNA annealig and ligation
3. Biochemistry
4. Cell biology
AAV
AAV Transfection Protocol
AAV精製
Coating glass surface
Giemsa stain
HaloTag染色
Lipofection
Passage of cultured cells
poly-Hemaコーティング
5. Microscopy, imaging
TOKAIHIT gas regulator
6. Software, programming, image analysis
Benchling
gRNA design
CellDesigner
Tips for CellDesigner
ImageJ/Fiji
Edit Lut
Tips for ImageJ/Fiji
Tracking by TrackMate in ImageJ/Fiji
MATLAB
FCS/FCCS analysis by MATLAB
MATLABのFCS/FCCS解析プログラム
FCCSanalysis_PCNC140805スクリプト
FCCSanalysis_PCNCKdスクリプト
Func_AcXcFit関数
Func_correlation関数
Func_detrend関数
Func_dirNumber関数
Func_Fitting関数
Func_tifToTimecourse関数
Image processing by MATLAB
FRETImageProcess2クラス
Adjust
AlignStack
CheckIntensityAroundNucleus
FigureBackground
FRET画像解析例
GetImage
GetTimeOfImage
IntensityHistogram
ModifySortArray
Movie
MovieFromTiff
OutputCAAXIntensity
OutputNESIntensity
OutputNLSIntensity
RatioImage
RatioImageFromTiff
ReadNDFile
ReadTiffFile
RegionForOutput
RegionGrowing
Segmentation
SortNLSNESCAAX
ソースコード
Tiffファイルの扱い方
TrackMate2MATLAB
基本事項
画像の表示と出力
顕微鏡画像読み込み
Tips for MATLAB
7. Python
Environment setup
Find RE site from AA sequence by Python program
Memo
Virtual environment
Visualization
Grid of Subplots
8. Others
Edit Lut
ImageJでLUTを編集
ExcelでR、G、Bの3列、256行の行列を作る。上が0、下が255
Control + L で Edit Lut as Text
LUTのテキストのところにExcelで作った行列をコピペ
Lookup Table>Set Lookup Tableで適用する
Image>Color>Edit LUTで確認。Saveで.lutファイルを保存
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