See attached PDF.

Pre-lab preparation:

1. Get an abbreviated copy of the lab in class from Dr. Kostenko.

2. In the extended online packet, read through the Background Information (p.4-9) and Procedure for Investigation 2.

4. The solutions we will have in class are 1M NaCl, 1M Sucrose, 1M Glucose, and Distilled water.

5. Formulate a hypothesis (i.e.: If solution X is inside the dialysis tubing and solution Y is outside the dialysis tubing, then the net movement of water will be, because..., and the cell will increase/decrease in mass.)

Lab Day:

1. You will work in groups of 2 and will be assigned a pair of solutions.

2. Compare hypotheses and come up with a unified "best plan."

3. Follow the procedure for Investigation 2 with the directions provided.

4. Execute the experiment during the double period. Record data for your group AND for the class.

5. When finished, break down your experiment, and clean up.

6. Immediately begin writing up a formal lab report in your lab notebook. Include graphs and answers to the appropriate study/post-lab questions.

This lab report MUST include data for THE WHOLE CLASS.

The analysis MUST include a graph and statements that incorporate findings from THE WHOLE CLASS.

The conclusion should incorporate answers to the following study questions on page 16 of the handout:

#1 in investigation I. Relate your answer to the lab we did. #1, 2, and 4 in investigation II.

7. Turn your lab notebook by the assigned date for review by Dr. Kostenko.

*If absent on lab day use the following website simulation: https://video.esc4.net/video/assets/Science/Biology/Gateway%20Resources/cell%20homeostasis%20virtual%20lab%20-%20activity/index.html

Click through the cell homeostasis virtual lab and record your data.

For the lab report include:

1. title and date

2. problem

3. hypothesis (see step 5 in pre-lab above)

4. experimental design

5. materials (had this been done in real life)

6. procedure (had this been done in real life)

7. data - in the form of a table

8. analysis - graph of the above results and answers to the questions after the simulation.

9. conclusion - talk about the experiment as a whole, what you found, and how it relates to cell homeostasis and movement of substances across a membrane.