👁️‍🗨️ Watch this to understand the whole process of Southern Blotting:  

1. Restriction Digest (0:22)---> DNA of various size produced 

2. Gel electrophoresis (1:30)--->  separate fragments by size to produce unique banding pattern.

3. Southern Blotting (3:10)---> denature DNA from double stranded to single stranded using alkaline solution--> use capillary action to transfer DNA from gel to nitrocellulose membrane. ---> band positions on membrane will match position on gel. 

4. Nucleic acid hybridisation (6:22)---> expose nitrocellulose membrane to single stranded radioactive gene probe that is complementary to sequence of interest. ---> probe will bind via hydrogen bonding to DNA fragments with complementary sequence.  

5. Autoradiography/fluorescent labelling (8:41) ---> pattern of hybridisation is visualised on X-ray film/fluoresce

👁️‍🗨️ Watch the video to understand the principles of nucleic acid hybridisation. As you watch, learn more about the following terms: 

1. hybridisation

2. probe 

3. examples of probe labels