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LEARNING OBJECTIVE:
To understand how different cell components can be separated and isolated for further studies.
Cell fractionation is the disruption of a cell and separation of its organelles by differential centrifugation.
The separation of organelles is based on size and density. Larger and denser organelles pellet at lower centrifugal forces.
How cell fractionation is done
How cell fractionation is done
👁️🗨️Watch the video that describes and explains how cell fractionation (involving two main steps - homogenization and ultracentrifugation) is done.
What are the conditions that the cells must be in before they are broken apart?
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