Embedded Files
RELEVANT LEARNING OUTCOME:
(a) Describe the principles and procedures of these molecular techniques: Southern blotting and nucleic acid hybridisation.
State the role of restriction enzymes in overall procedure to identify gene of interest.
🕐Estimated time for this section: 15 minutes
Restriction Enzymes
Restriction Enzymes
Watch the animation (x1.5 speed) to find out more about restriction enzymes:
What are the functions of the two enzymes that restrict the growth of bacteriophage in E.coli?
What is the name given to the specific nucleotide sequences that are recognized by restriction endonucleases?
What is meant by palindromic sequence?
After digestion with restriction enzymes, how can scientists identify which DNA fragment contains the gene of interest? (relate to Southern Blotting)
Sticky & blunt ends
Sticky & blunt ends
Diagram on the left show how blunt ends and sticky ends are produced by different restriction enzymes
🖊️Check Your Understanding: Learning Goals
🖊️Check Your Understanding: Learning Goals
Attempt Qn 19-20 of the Molecular techniques Learning Goals
Page updated
Google Sites
Report abuse