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RELEVANT LEARNING OUTCOME:
(b) Investigate and explain the effects of temperature, pH, enzyme concentration and substrate concentration on the rate of an enzyme-catalysed reaction by measuring rates of formation of products (e.g. measuring gas produced using catalase) or rate of disappearance of substrate (e.g. using amylase, starch and iodine).
Factors that affect enzyme-catalysed reaction
Factors that affect enzyme-catalysed reaction
๐๏ธโ๐จ๏ธ Watch the video to understand how temperature and pH affect the rate of enzyme-catalysed reaction
Effect of substrate concentration on rate of reaction
Effect of substrate concentration on rate of reaction
๐๏ธโ๐จ๏ธ Watch the video to understand how substrate concentration affects the rate of enzyme-catalysed reaction. The concentration of enzymes have similar effect and hence same general shape of graph.
At low substrate concentration, as substrate concentration increase, rate of enzyme-catalysed reaction increases. More enzyme-substrate complexes are formed per unit time resulting in more products formed per unit time. The limiting factor is concentration of substrate.ย
At high substrate concentration, any increase in substrate concentration will not increase rate of reaction. Active site of enzymes are now saturated with substrate molecules.
๐๏ธCheck Your Understanding: Lecture notes Checkpoint
๐๏ธCheck Your Understanding: Lecture notes Checkpoint
The limiting factors are indicated on the different sections of the following two graphs.ย
Take note of the x-axis of both graphs.
Recall: Limiting factor directly affects the biochemical process if its quantity is changed.
2. Draw a graph to show how temperature would affect the rate of reaction of an enzyme that has an optimal temperature of 65ยฐC.
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