RELEVANT LEARNING OUTCOME:
(a) Describe how the information on DNA is used to synthesise polypeptides. (Description of the processes of transcription, formation of mRNA from pre-mRNA and translation is required.)
Watch the video to observe the process of transcription, which includes initiation, elongation and termination. From 1:18 onwards is the process of post-transcriptional modification.
TATA box in promoter of template DNA strand
General transcription factors (GTF) and RNA polymerase (RNA pol)
Transcription initiation complex: GTF, RNA pol, promoter
RNA pol move in a 3' to 5' direction
new RNA strand is synthesised in a 5' to 3' direction
Free ribonucleotides match via complementary base pairing
transcription stop site
primary RNA transcript (in eukaryotes) and mRNA (in prokaryotes)
polyadenylation signal sequence transcribed (AAUAAA) in eukaryotes (in prokaryotes, it is terminator RNA sequence)
The simulation below shows how the process of transcription occurs. Click on the complementary RNA nucleotide to complete the transcription process.
Attempt section 2, Qns 7 - 14 of this SLS lesson.