jsmc-10068

SEROPREVALENCE OF CHLAMYDIA PNEUMONIAE ANTIBODIES AMONG CHILDREN ADMITTED FOR RESPIRATORY INFECTIONS IN SULAIMANI PEDIATRIC TEACHING HOSPITAL

Alan Abdula Abdulrahman *, Ali Hattem Hussain **, Nasih Othman ** and Mohammed Ibrahim M. Gubari **

*   Department of Pediatrics, School of Medicine, Sulaimani University, lecturer, Sulaimani, Iraq.

** Department of Community Health, Sulaimani Polytechnic University, Sulaimani, Iraq.

Submitted: 6/9/2014; Accepted: 1/12/2014; Published 1/6/2015

DOI Link: https://doi.org/10.17656/jsmc.10068 

ABSTRACT

Background

The incidence of severe acute respiratory tract infections in children caused by Chlamydia pneumoniae varies greatly from year to year and place to place around the world. Chlamydia pneumoniae, a bacterium that causes respiratory infections, is probably under-diagnosed, it’s of worldwide distribution. The seroprevalence of C.

pneumoniae antibody is age dependent.

Objectives

This study examined the prevalence of C. pneumoniae infections, their clinical manifestations and demographic features in hospitalized children with respiratory tract infections. The study also tested two different immunological techniques for detection C. pneumoniae infections.

Methods

This cross sectional study was carried out in Sulaimani Pediatric teaching hospital. One hundred and twenty seven children aged less than 5 years, hospitalized for respiratory tract infections were enrolled in this study. Serum anti-C. pneumoniae specific IgM and IgG antibodies were measured using an enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent assay technique (IFA). The clinical and demographic data were compared between positive C. pneumoniae antibodies and negative C. pneumoniae antibodies.

Results

Acute C. pneumoniae infections were detected using IgM anti-C. pneumoniae antibodies which were positive in 5 (3.9%) children by ELISA technique and the same result was obtained by IFA technique. IgG anti-C. pneumoniae antibodies were positive in 32 (25.2%) and 33 (25.9%) by ELISA and IFA respectively, the results of the two tests were similar and the differences were statistically not significant. Clinical and demographic data of patients with C. pneumoniae infections were comparable to those of negative IgM anti-C. pneumoniae antibodies.

Conclusion

C. pneumoniae has a role in children hospitalization with respiratory tract infections in Sulaimani Governorate. ELISA technique is a good alternative for IFA in serological diagnosis of C. pneumoniae infections.

KEYWORDS

Hospitalized children, Anti-Chlamydia pneumoniae antibodies, ELISA technique, IFA technique, respiratory tract infections.

References 

1. Puolakkainen M. Laboratory diagnosis of persistent human chlamydial infection. Front Cell Infect Microbiol. 2013;17:93-99.

2. Everett KD, Bush RM, Andersen AA. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int J Syst Bacteriol. 1999; 49:415–440.

3. Specjalski K. Role of Chlamydia pneumoniae and Mycoplasma pneumoniae infections in the course of asthma. Pneumonol Alergol Pol. 2010;78:284-295.

4. Sakurai-Komada N, Koike KA, Kaku Y, Hiraki M, Cui R, Sankai T, et al. Chlamydia pneumoniae infection was associated with risk of mortality from coronary heart disease in Japanese women but not men: the JACC Study. J Atheroscler Thromb. 2010; 17(5):510-516.

5. Normann E, Gnarpe J, Gnarpe H, Wettergren B. Chlamydia pneumoniae in children with acute respiratory tract infections. Acta Paediatr. 1998; 87(1):23-7.

6. Paldanius M, Bloigu A, Alho M, Leinonen M, Saikku P. Prevalence and Persistence of Chlamydia pneumoniae Antibodies in Healthy Laboratory Personnel in Finland. Clin. Diagn. Lab. Immunol. 2005; 12:654-659. 

7. She RC, Thurber A, Hymas WC, Stevenson J, Langer J, et al. Limited utility of culture for Mycoplasma pneumoniae and Chlamydophila pneumoniae for diagnosis of respiratory tract infections. J Clin Microbiol. 2010;48:3380-33382.

8. Otomo S, Yamamura J, Hayashi E, Nakamura T, Kakinuma H, et al. Analysis of children with Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae respiratory infections by real-time PCR assay and serological tests. APMIS. 2008;116:477-483.

9. Miyashita N., Saito A., Kohno S., Oizumi K, Yamaguchi K et al. Community-acquired Chlamydia pneumoniae pneumonia in Japan: a prospective multicenter community-acquired pneumonia study. Intern Med. 2002; 41:943-949.

10. Choroszy-Król I, Frej-Mądrzak M, Hober M, Sarowska J, Jama-Kmiecik A. Infections Caused by Chlamydophila pneumoniae. Adv Clin Exp Med. 2014;23:123-126.

11. Choroszy-Krol I, Frej-Madrzak M, Jama-Kmiecik A, Sarowska J,  Gosciniak G, et al. Incidence of Chlamydophila Pneumoniae Infection in Children During 2007–2010. Neurobiology of Respiration Adv Exp Med Biol. 2013;788:83-87.

12. Chen Z, Ji W, Wang Y, Yan Y, Zhu H, et al. Epidemiology and associations with climatic conditions of Mycoplasma pneumoniae and Chlamydophila pneumoniae infections among Chinese children hospitalized with acute respiratory infections. Ital J Pediatr. 2013;25:34-39.  

13. Kurz H, Gopfrich H, Wabnegger L, Apfalter P. Role of Chlamydophila pneumoniae in children hospitalized for community-acquired pneumonia in Vienna, Austeria. Pediatr Pulmonol 2009; 44:873-876.

14.  Schmidt SM, Müller CE, Krechting M, Wiersbitzky H, Gürtler L, Wiersbitzky SK,  et al. Chlamydia pneumoniae carriage and infection in hospitalized children with respiratory tract diseases. Infection. 2003;31:410-416.

15. Schmidt SM, Muller CE, Mahner B, Wiersbitzky SK. Prevalence, rate of persistence and respiratory tract symptoms of Chlamydia pneumoniae infection in 1211 kindergarten and school age children. Pediatr Infect Dis J. 2002;21:758-762.

16. Othman N, Isaacs D, Kesson A. Mycoplasma pneumoniae infections in Australian children. J Paediatr Child Health 2005; 41:671-676. 

17. Bosnak M, Dikici B, Bosnak V, Dogru O, Ozkan I, Ceylan A, et al. Prevalence of Mycoplasma pneumoniae in children in Diyarbakir, the south-east of turkey. Pediatr Int. 2002; 44:510-512. 

18. Higashigawa M, Kawasaki Y, Yodoya N. Prevalence of Mycoplasma IgM in children with lower respiratory tract illness. Pediatr Int. 2009; 51:684-686.

19. Vainas T, De Graaf R, Stassen FR, Kurvers HA, Grauls GE, Kitslaar PJ, et al. Chlamydia pneumoniae serology: comparing a commercial enzyme immunoassay and microimmunofluorescence test in patients with cardiovascular disease. APMIS. 2003 Feb; 111:363-369.

20. Dowell S. F, Peeling R. W, Boman J, Carlone G. M , Fields B. S, Guarner J, et al. The Chlamydia pneumoniae Workshop Participants. Standardizing Chlamydia pneumoniae assays: recommendations from the Centers for Disease Control and Prevention (USA) and the Laboratory Centre for Disease Control (Canada). Clin. Infect. Dis. 2001; 33:492-503. 

21. Kuo, C. C,  Jackson L. A, Campbell L. A, Grayston J. T. Chlamydia pneumoniae (TWAR). Clin. Microbiol. Rev.1995. 8:451-461. 

22. Grassi T, Mancini F, Ciervo A, Vescio MF, Ghazal A, Ashur H, et al. Chlamydophila pneumoniae, Mycoplasma pneumoniae and influenza in children with respiratory infections in Alexandria, Egypt. J Infect Dev Ctries. 2014; 8:379-383.

23. Kumar S,  Saigal R. S, Sethi R. G. Detection of IgM and IgG antibodies to Chlamydophila pneumoniae in pediatric community-acquired lower respiratory tract infections. Indian J Pathol Microbiol. 2011;54:782-785. .

24. Tuuminen T, Varjo S, Ingman H, Weber T, Oksi J, Viljanen M. Prevalence of Chlamydia pneumoniae and Mycoplasma pneumoniae Immunoglobulin G and A Antibodies in a Healthy Finnish Population as Analyzed by Quantitative Enzyme Immunoassays. Clin Diagn Lab Immunol. 2000;7: 734–738.

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