jsmc-10038

EFFECT OF ACYCLOVIR ON EMBRYO IMPLANTATION IN MICE

Snoor Jalal Mustafa * and Kameel Mate Naoum **

* Department of Anatomy and Histology, School of Medicine, Faculty of Medical Sciences, University of Sulaimani, Sulaimani-Kurdistan Region of Iraq.

** Department of Anatomy and Histology, College of Medicine, Hawler Medical University, Erbil-Iraq.

Submitted: 27/4/2013; Accepted: 5/8/2013

DOI Link: https://doi.org/10.17656/jsmc.10038

ABSTRACT

Background

Acyclovir is the antiviral drug used for treatment of herpes simplex and varcilla zoster viral infections and its adverse effects are not well studied on the embryo implantation.

Objectives

This study was conducted to determine the effect of Acyclovir on the process of implantation in mice of Swiss Webster Albino type and to detect whether there is any effect of Acyclovir on implantation of embryo in mice.

Materials and Methods

Adult female Swiss Webster albino mice were used in this study, aged 8-10 weeks and of 20-25gms body weight. The study was conducted from October 2009 through January 2010. A group of 24 mature female mice were included in this study and they were divided into two groups: Group 1(experimental group), included 12 mice injected with a single daily dose of 50 mg/kg b.wt. of Acyclovir subcutaneously on day 1-5 of gestation at 10:00 a.m; Group 2 included 12 mice injected D.W subcutaneously and considered as a control group. All animals were sacrificed on day 7 at noon and the uteri of all mice were examined for presence of implantation sites.

Results

In this study, out of 12 pregnant females, only two embryos were implanted from only one mouse (8.33%), the remaining 11 (91.67%) showed no sign of implantation at day7 postcoitus. The mean implant in the experimental group was 0.16±0.75, whereas in the control group a total of 84 embryos from 12 pregnant females were implanted and the mean implant was 7±1.41. Statistically, the result was highly significant since the p-value was < 0.01.

Conclusion

Exposure to Acyclovir at early days of pregnancy resulted in inhibition of embryo implantation.

KEYWORDS

Acyclovir, Implantation, Embryo, Mice.

References

1- Enders A .A morphological analysis of the early implantation stages in rat. Am.J. Anat. 1976; 125: 1-29.

2- Paria B.C, Huet Y.,Hudson, and DeyS.K. Blastocyst's state of activity determines the "window" of implantation in the receptive mouse uterus. Proc Natl Acad Sci.1993 ; Nov 1. 90 (21):10159-62.

3- De Clercq, Erik, and Field Hugh J .Antiviral prodrugs- the development of successful prodrug strategies for antiviral chemotherapy. British Journal of Pharmacology.2006; 147:1 -11.

4- Stray –Pedersen B. Acyclovir in late pregnancy to prevent neonatal herpes simplex. Lancet. 1990; 336: 756.

5- Hale T. Medication and mother milk .9th ed. Amarillo, TX, Pharmasoft Publishing:2000.

6- Brigden D, Rosling AE, andWoods NC .Renal function after Acyclovir intravenous injection. The American Journal of Medicine.1982; 73 (1A): 182 -185.

7- Landsburger EJ, Hager WD, and Grossman JH. Successful management of varcilla pneumonia complicating pregnancy . A report of three cases. J Reprod Med.1986;31 :3114.

8- Henderson GI, Huz-Q, Johnson RE, Perez AB, Yang Y, and Schenker S. Acyclovir transport by the human placenta .J Lab Clin Med.1992 ;120 :885-92.

9- Glaxo Smith Kline Zovirax capsule, tablet , and suspension. Prescribing information; 2007.

10- Stephan K,Constanze L, Gurdrum B; Heas J and Diether N. Effect of Acyclovir on mammalian embryonic development in culture. Arch of Toxicol.1995; 58(2) :89 -96.

11- Chahoud I, Stahlmann R, Boochert G, Dillmann I, and Neubert D. Gross structural defects in rats after Acyclovir application on day 10 of gestation. Arch Toxicol. 1988;62 :8 -14.

12- Furmann PA, de Miranda P, St. Clair MH, and Elion B.B. Metabolism of Acyclovir in virus –infected cell. Antimicrob Agent Chemother.1981; 20: 518 -524.

13-Schneider HA. Nutritional factors in host resistance. Bacteriol.1956; 24:186-19.

14- Sepctor WB. Handbook of Biological data. Nat. Res.Counc-Nat Acad. Sci. Saunders, Philadelphia. 1986; P.584.

15- Everett JW. Progesteron and estrogen in the experimental control of ovulation time and other features of the estrus cycle in the rat . Endocrin .1984 ; 43:389-405.

16- Orsini M.W. Technique of preparation, study and photography of benzyl-benzoate cleared material for embryological studies. J.Reprod.and Fertility.1992; 3: 283-287.

17- Duc-Goiran P, Mignot TM, Bourgeois C, and Ferré F. Embryo-maternal interactions at the implantation site: a delicate equilibrium.Eur. J.Obs.Gynaeco.Reprod.Biol. 1999 Mar; 83(1):85-100.

18- Finn CA and Martin L. The control of implantation. J Reprod Fertil.1974; 39:195–206.

19- Yoshinaga K Uterine receptivity for blastocyst implantation. Ann N Y Acad Sc..1988; 54: 424–431.

20- Paria BC, Ma W, Tan J, Raja S, Das SK, Dey SK, and Hogan BL.Cellular and molecular responses of the uterus to embryo implantation can be elicited by locally applied growth factors, PNAS.2001; 98 1047-1052.

21- Mamede J.A., Simoes M.D.J., Nono N.F., Juliano R.M., Oliveira-Fihlo, and Kulay L. Chronic effect of Azidothymidine and Acyclovir on pregnant rats. Gen. Pharmacol. 1995; 26: 523-526.

22- Moor HL, Szczech GM, Rodwell DE , Kapp RW, de Miranda P ,Tucker WE Preclinical toxicology studies with Acyclovir , teratogenic, reproductive, and neonatal tests. Fund Appl. Toxico. 1983; 3: 5608.

23 - Kazem Parivar, Homa Mohseni kouch esfahani, and Baharak Mohammad zadeh asl .Surveying of topical Acyclovir side effects on developing embryos of Balb/C mice strain. J. Biology. 2006; 5: 2-13.

24- De Miranda P, Krasny H.C, Page D.A, and Elion G.B. Species difference in the disposition of Acyclovir. Am. J.Med. 1982; 73: 31-35.

25- Tucker JR, WE,Macklin AW, Scot RJ,Johnson RE, Elion GB,de Miranda P., and Szczech GM. Preclinical toxicology studies with Acyclovir: Acute and Subchronic tests. Fund Appl. Toxicol. 1983; 3: 573-578.

26- Stern, S., J. D. Biggers, and E. Anderson. Mitochondria and early development of the mouse. J. Exp. Zool. 1978; 176:179-192.

27- Calarco, P. G., and E. H. Brown. An ultrastructural and cytological study of preimplantation development of the mouse. J. Exp. Zool. 1999; 171:253-284.

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