Microbiology with Diseases by Taxonomy Chapter 4 Answers

Microbiology with Diseases by Taxonomy Chapter 4 Answers

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Microbiology with Diseases by Taxonomy Chapter 4 Answers 1CM

Gram staining is a method that is used to differentiate bacterial species into gram-negative and gram-positive.This method was developed by Hans Christian Gram.

This staining method differentiates bacteria by the physical and chemical characteristics of the bacterial cell walls.

In this method a primary stain, a mordent (iodine), decolorizer and counter stain is used. A gram-positive bacterium appears in purple-blue color while gram-negative appears in pinkish-red color after staining.

Microbiology with Diseases by Taxonomy Chapter 4 Answers 1CT

Miss M. did a Gram stain in her practical class and came home with green fingers, and a low grade.

M must have mistakenly used another stain instead of crystal violet for the primary stain. The stain she used might have been malachite green. Malachite green is usually used in spore staining but not in Gram staining.

The procedure of Gram staining is:

1. A smear is made with culture on a glass slide.
2. The smear is air dried and heat fixed.
3. The smear is then flooded with the primary stain.