ALiX is developed for basic research on diffusing two-color single-molecule FRET in single or multiple spot geometries.
To get an overview of the software capability, please check the ALiX Overview page of the manual.
The ALiX Workflow page provides a bird's eye view of the different steps involved in the analysis of a data file.
Tutorial pages will be added in the future, using example files from published papers to illustrate typical analysis workflows.