Analysis menu

The Analysis menu gives access to functionalities that would require one or more click on one or several buttons in the different pages they refer to. In this sense, they can be thought of as shortcuts for macros.

Let's review the actions performed by each of these menu items, shown in the Figure below.

1. Alternation Period Definition (Ctrl+P)

When alternation periods parameters (start and stop of each excitation or emission period) have already been defined by the user in either the us Alternation Periods or Nanotime Display page, performing this action uses them to flag each photon as belonging to the Donor or Acceptor period, or neither (in which case they are flagged as belonging to an "Undefined" period).

This skips the histogramming step and is equivalent to pressing the Define Periods button of the us Alternation Periods page or the Periods button of the Nanotime Display page.

2. Compute Nanotimes (Ctrl+Shift+N)

In the case of ns ALEX data, once the emission periods have been defined in the Nanotime Display page, the nanotime stamp of each photon can be converted into a nanotime defined with respect to its period's start time. This steps is mandatory in order to compute any burst statistics involving nanotimes.

3. Background Rate MLE (Ctrl+M)

This is equivalent to pressing the Bkgd R MLE button in the Background Analysis page. This action computes photon intervals and an MLE estimate of the mean interval between consecutive photons in each stream.

4. Background Rate Analysis (Ctrl+B)

This action is equivalent to successively pressing the PIH (Photon Interval Histogram), Fit (Photon Interval Histogram Fit) and the Accept all displayed rates buttons on the Background Analysis page (or, when the Background Rate Used for Burst Search pull-down list shows MLE Rate, this action is equivalent to pressing Bkgd R MLE and Accept all displayed rates).

5. Advanced Background Rate Analysis (Ctrl+Shift+B)

This action is equivalent to pressing the Adv. Background button of the Advanced Background Analysis page.

6. Burst Count Rates (Ctrl+R)

This action is equivalent to pressing the Burst Count Rates button of the Count Rate Analysis page. This step is mandatory to be able to use these quantities in burst statistics.

7. Burst Search (Ctrl+S)

This action is equivalent to pressing the Burst Search button of the Burst Search page.

8. Burst Analysis (Ctrl+A)

This action is equivalent to pressing the Burst Analysis button of the Burst Statistics Definitions page (a tab of the Burst Analysis page).

9. ALEX Histogram (Ctrl+L)

This action is equivalent to pressing the ALEX Histogram button of the ALEX Analysis page.

10. ACF Computation (Ctrl+F)

This action is equivalent to pressing the ACF button of the Correlation page.

11. CCF Computation (Ctrl+Shift+F)

This action is equivalent to pressing the CCF button of the Correlation page.

12. All Analysis Steps (Ctrl+Shift+A)

This action is equivalent to successively performing all actions described above up to ALEX Histogram (in that order).

Note that if a checkbox instructing the software to switch to another page is checked on any given page, this action will also be performed. It is therefore recommended to uncheck all Switch to... checkboxes to avoid page switching while analysis proceeds automatically.

13. Load File + All Analysis Steps (Ctrl+Shift+O)

This action is equivalent to loading the file whose path is selected in the File Path control of the Data File page in memory and then performing All Analysis Steps.

14. General Multispot Analysis (Ctrl+M)

This action is equivalent to presing the General Multispot Analysis button in the Multispot Analysis tab of the Scripting page.

Note that this feature can also be used with single-spot data, and therefore allows performing different actions than the previous two scripts.

15. Clear All Data

This action clears all the internally saved data (such as photon and burst data, intermediate arrays and histograms). This can help with releasing some memory for other applications.