Multispot Analysis

• Process all files in Data Folder

If this box is checked (and a valid folder path is provided in the Data Folder control), any selected script will be applied to each of the valid data files in that folder.

Note that if you opt to Clear All Burst Analysis Graphs Before Start, by checking the corresponding box, or if you have checked the Clear Chart Before Script box in the Monitored Variables tab, or if you have checked the Reset All Graphs to Default when Loading a New File box in ALiX Settings>>General Settings, these options will be applied for each file.

• Use All/only Selected Channels

The Use All/only Selected Channels switch specifies whether or not all channels listed on the Data File page should be used during the script. If this is the case, some analyses using channel pairs require further specifications of the pairs to be analyzed. This is defined by the Channel Pairs (D, A) list (a string), as discussed next.

If not all channels are to be used (i.e. the switch is on the Use only Selected Channels position), the list of channel pairs will respect this choice. For instance, if the list contains the pair (0,1), but channel 0 is not selected, then that pair will not be formed and this analysis step will be skipped (or return null results).

• Channel Pairs (D, A)

The list of channel pairs can have different formats, and specifies a Donor (D), followed by an Acceptor (A) channel. This definition will override any previous definitions of these channels.

A typical list would be:

1,2

1,3

1,4

...

where the format is:

A,B<newline>

A: First channel name (as loaded from file, not necessarily a number, as illustrated below),

B: Second channel name.

Space characters will be ignored.

Other valid list syntax will be introduced in the discussion of the CCF calculation script.

Note: Although this tab is entitled Multispot Analysis, the scripts can also be run with single-spot data files.