Genetics: micro-array (Joshua Junkermeier)

Title: Micro-array: investigating causes of cancer ("cabbage version" - modified from NOVA© version)

Science and Engineering Practices (NGSS): (as numbered in link) #1, #3, #4, #6, #7, and #8

High School Standards (middle school also applies: HS-LS3-1 and HS-LS3-2

Materials: This lab is followed as well as modified from NOVA Teachers

Procedure: 1) The procedures are based on NOVA's Teacher's Guide

2) The procedures for the "cabbage" version of lesson. This version modifies materials in order to

improve the interpretation of results

Student prior knowledge: Student's should be familiar with at least heredity phenomenon, Mendelian Genetics, and may

need an initial understanding of DNA''s structure as a polymer

Explanation: Use the link to review NOVA's Teacher Guide. However, the rationale behind the lesson activity,

modifications of the lesson, self-made supplementary materials, and scope and sequence are discussed.

Questions & Answers: Pre-assessment questions and Data Analysis questions

Applications to Everyday Life:

1. Understanding Genetic-Technology's role in medical treatment.
2. Understanding Civic roles of society regarding use of genetic technology.
3. Understanding the role of environment on an individuals health and genotype.

Photographs:

image 1: materials (NaCl solution, distilled H2O, vinegar, image 2: Microarray ready for patients' 2 "DNA sample"

and ammonia (used in "cabbage" microarray only) (DNA = phenophthalein solution)

image 3: "DNA sample" has been added to each reservoir. image 4: interpreting results can be difficult. While

See key to interpret results. All microarray equipment un-anticipated results should still allow students to

must be thoroughly clean from prior use to avoid any engage in guided inquiry and complete lesson, the

false positive results due to residue remaining from results will not match the NOVA key. Moreover,

a prior lab. the results may prevent a "safe" medication to be

prescribed. Within a CSCS-lesson framework,

multiple groups may need to engage in argument

as to the cause of experimental error and false-

positive interpretation of gene activity.

Videos:

1. Video 2: a tutorial covering pre-lab activity of the methods and mechanics of the micro-array procedure.
2. Video 3: a tutorial covering data collection as well as guidance on supplementary tasks (a non-CSCS version of lesson: structured inquiry using online resources)
3. Anyone interested in materials can explore this shareable file or email: joshuajunkermeier@burbankusd.org